Journalartikel
Autorenliste: Mehta, Aditi; Cordero, Julio; Dobersch, Stephanie; Romero-Olmedo, Addi J.; Savai, Rajkumar; Bodner, Johannes; Chao, Cho-Ming; Fink, Ludger; Guzmán-Díaz, Ernesto; Singh, Indrabahadur; Dobreva, Gergana; Rapp, Ulf R.; Günther, Stefan; Ilinskaya, Olga N.; Bellusci, Saverio; Dammann, Reinhard H.; Braun, Thomas; Seeger, Werner; Gattenlöhner, Stefan; Tresch, Achim; Günther, Andreas; Barreto, Guillermo
Jahr der Veröffentlichung: 2016
Seiten: 1380-1389
Zeitschrift: EMBO Molecular Medicine
Bandnummer: 8
Heftnummer: 12
ISSN: 1757-4676
eISSN: 1757-4684
Open Access Status: Gold
DOI Link: https://doi.org/10.15252/emmm.201606382
Verlag: Springer
Abstract:
Lung cancer (LC) is the leading cause of cancer-related deaths worldwide. Early LC diagnosis is crucial to reduce the high case fatality rate of this disease. In this case-control study, we developed an accurate LC diagnosis test using retrospectively collected formalin-fixed paraffin-embedded (FFPE) human lung tissues and prospectively collected exhaled breath condensates (EBCs). Following international guidelines for diagnostic methods with clinical application, reproducible standard operating procedures (SOP) were established for every step comprising our LC diagnosis method. We analyzed the expression of distinct mRNAs expressed from GATA6 and NKX2-1, key regulators of lung development. The Em/Ad expression ratios of GATA6 and NKX2-1 detected in EBCs were combined using linear kernel support vector machines (SVM) into the LC score, which can be used for LC detection. LC score-based diagnosis achieved a high performance in an independent validation cohort. We propose our method as a non-invasive, accurate, and low-price option to complement the success of computed tomography imaging (CT) and chest X-ray (CXR) for LC diagnosis.
Zitierstile
Harvard-Zitierstil: Mehta, A., Cordero, J., Dobersch, S., Romero-Olmedo, A., Savai, R., Bodner, J., et al. (2016) Non-invasive lung cancer diagnosis by detection of GATA6 and NKX2-1 isoforms in exhaled breath condensate, EMBO Molecular Medicine, 8(12), pp. 1380-1389. https://doi.org/10.15252/emmm.201606382
APA-Zitierstil: Mehta, A., Cordero, J., Dobersch, S., Romero-Olmedo, A., Savai, R., Bodner, J., Chao, C., Fink, L., Guzmán-Díaz, E., Singh, I., Dobreva, G., Rapp, U., Günther, S., Ilinskaya, O., Bellusci, S., Dammann, R., Braun, T., Seeger, W., Gattenlöhner, S., ...Barreto, G. (2016). Non-invasive lung cancer diagnosis by detection of GATA6 and NKX2-1 isoforms in exhaled breath condensate. EMBO Molecular Medicine. 8(12), 1380-1389. https://doi.org/10.15252/emmm.201606382
