Journal article

Authors list: Jakob, Kevin; Schwack, Wolfgang; Morlock, Gertrud E.

Publication year: 2024

Journal: Food Chemistry

Volume number: 453

ISSN: 0308-8146

eISSN: 1873-7072

Open access status: Hybrid

DOI Link: https://doi.org/10.1016/j.foodchem.2024.139593

Publisher: Elsevier

Abstract: 
Ergot alkaloids, naturally occurring mycotoxins of Claviceps fungi, pose health risks. This necessitates accurate analysis methods to ensure food safety. This study explored the open-source miniaturized all-in-one 2LabsToGo system to analyze ergot alkaloids in whole rye samples. It is suited for sustainable atline analysis as it combines all planar chromatography tasks, allowing low-cost quality control in milling plants. The LOD and LOQ of ergocristine were determined to be 0.4 and 1.2 ng/zone, respectively. Detectability of ergot alkaloids was proven to be below the current maximum limit of 500 mu g/kg for rye milling products. The repeatability ( %RSD ) was 4.1 % and the coefficient of determination of the analytical response (R 2 ) was 0.9918 for ergocristine. The mean recovery rate of ergot alkaloids in spiked whole rye grain was close to 100 %. Results of screening whole rye for ergot alkaloids were successfully verified by comparison with those obtained by conventional status quo HPTLC instrumentation.

Harvard Citation style: Jakob, K., Schwack, W. and Morlock, G. (2024) All-in-one 2LabsToGo system for analysis of ergot alkaloids in whole rye, Food Chemistry, 453, Article 139593. https://doi.org/10.1016/j.foodchem.2024.139593

APA Citation style: Jakob, K., Schwack, W., & Morlock, G. (2024). All-in-one 2LabsToGo system for analysis of ergot alkaloids in whole rye. Food Chemistry. 453, Article 139593. https://doi.org/10.1016/j.foodchem.2024.139593