Journalartikel

Jahr der Veröffentlichung: 2024

Zeitschrift: Theoretical and Applied Genetics

Bandnummer: 137

Heftnummer: 6

ISSN: 0040-5752

eISSN: 1432-2242

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1007/s00122-024-04634-9

Verlag: Springer

Abstract: 
The sequence diversity of three homoeologous copies of the PHOTOPERIOD-1 gene in European winter wheat was analyzed by Oxford Nanopore amplicon-based multiplex sequencing and molecular markers in a panel of 194 cultivars representing breeding progress over the past 5 decades. A strong, consistent association with an average 8% increase in grain yield was observed for the PpdA1-Hap1 haplotype across multiple environments. This haplotype was found to be linked in 51% of cultivars to the 2NS/2AS translocation, originally introduced from Aegilops ventricosa, which leads to an overestimation of its effect. However, even in cultivars without the 2NS/2AS translocation, PpdA1-Hap1 was significantly associated with increased grain yield, kernel per spike and kernel per m(2) under optimal growth conditions, conferring a 4% yield advantage compared to haplotype PpdA1-Hap4. In contrast to Ppd-B1 and Ppd-D1, the Ppd-A1 gene exhibits novel structural variations and a high number of SNPs, highlighting the evolutionary changes that have occurred in this region over the course of wheat breeding history. Additionally, cultivars carrying the photoperiod-insensitive Ppd-D1a allele not only exhibit earlier heading, but also deeper roots compared to those with photoperiod-sensitive alleles under German conditions. PCR and KASP assays have been developed that can be effectively employed in marker-assisted breeding programs to introduce these favorable haplotypes.

Harvard-Zitierstil: Makhoul, M., Schlichtermann, R., Ugwuanyi, S., Weber, S., Voss-Fels, K., Stahl, A., et al. (2024) Novel PHOTOPERIOD-1 gene variants associate with yield-related and root-angle traits in European bread wheat, TAG Theoretical and Applied Genetics, 137(6), Article 125. https://doi.org/10.1007/s00122-024-04634-9

APA-Zitierstil: Makhoul, M., Schlichtermann, R., Ugwuanyi, S., Weber, S., Voss-Fels, K., Stahl, A., Zetzsche, H., Wittkop, B., Snowdon, R., & Obermeier, C. (2024). Novel PHOTOPERIOD-1 gene variants associate with yield-related and root-angle traits in European bread wheat. TAG Theoretical and Applied Genetics. 137(6), Article 125. https://doi.org/10.1007/s00122-024-04634-9