Journal article
Authors list: Lipskaia, Larissa; Breau, Marielle; Cayrou, Christelle; Churikov, Dmitri; Braud, Laura; Jacquet, Juliette; Born, Emmanuelle; Fouillade, Charles; Curras-Alonso, Sandra; Bauwens, Serge; Jourquin, Frederic; Fiore, Frederic; Castellano, Remy; Josselin, Emmanuelle; Sanchez-Ferrer, Carlota; Giovinazzo, Giovanna; Lachaud, Christophe; Gilson, Eric; Flores, Ignacio; Londono-Vallejo, Arturo; Adnot, Serge; Geli, Vincent
Publication year: 2024
Pages: 1650-1684
Journal: EMBO Reports
Volume number: 25
Issue number: 3
ISSN: 1469-221X
eISSN: 1469-3178
Open access status: Gold
DOI Link: https://doi.org/10.1038/s44319-023-00041-1
Publisher: Springer
Abstract:
Lung diseases develop when telomeres shorten beyond a critical point. We constructed a mouse model in which the catalytic subunit of telomerase (mTert), or its catalytically inactive form (mTert(CI)), is expressed from the p21(Cdkn1a) locus. Expression of either TERT or TERTCI reduces global p21 levels in the lungs of aged mice, highlighting TERT non-canonical function. However, only TERT reduces accumulation of very short telomeres, oxidative damage, endothelial cell (ECs) senescence and senile emphysema in aged mice. Single-cell analysis of the lung reveals that p21 (and hence TERT) is expressed mainly in the capillary ECs. We report that a fraction of capillary ECs marked by CD34 and endowed with proliferative capacity declines drastically with age, and this is counteracted by TERT but not TERTCI. Consistently, only TERT counteracts decline of capillary density. Natural aging effects are confirmed using the experimental model of emphysema induced by VEGFR2 inhibition and chronic hypoxia. We conclude that catalytically active TERT prevents exhaustion of the putative CD34 + EC progenitors with age, thus protecting against capillary vessel loss and pulmonary emphysema.
Citation Styles
Harvard Citation style: Lipskaia, L., Breau, M., Cayrou, C., Churikov, D., Braud, L., Jacquet, J., et al. (2024) mTert induction in p21-positive cells counteracts capillary rarefaction and pulmonary emphysema, EMBO Reports, 25(3), pp. 1650-1684. https://doi.org/10.1038/s44319-023-00041-1
APA Citation style: Lipskaia, L., Breau, M., Cayrou, C., Churikov, D., Braud, L., Jacquet, J., Born, E., Fouillade, C., Curras-Alonso, S., Bauwens, S., Jourquin, F., Fiore, F., Castellano, R., Josselin, E., Sanchez-Ferrer, C., Giovinazzo, G., Lachaud, C., Gilson, E., Flores, I., ...Geli, V. (2024). mTert induction in p21-positive cells counteracts capillary rarefaction and pulmonary emphysema. EMBO Reports. 25(3), 1650-1684. https://doi.org/10.1038/s44319-023-00041-1
Keywords
Capillary Density; DRIVEN; EMPHYSEMA; P21; QUIESCENCE; Telomerase; TELOMERE DYSFUNCTION
