Journal article

Publication year: 2024

Journal: FEMS Microbiology Ecology

Volume number: 100

Issue number: 3

ISSN: 0168-6496

eISSN: 1574-6941

Open access status: Gold

DOI Link: https://doi.org/10.1093/femsec/fiae023

Publisher: Oxford University Press

Abstract: 

Monitoring of bioinoculants once released into the field remains largely unexplored; thus, more information is required about their survival and interactions after root colonization. Therefore, specific primers were used to perform a long-term tracking to elucidate the effect of Hartmannibacter diazotrophicus on wheat and barley production at two experimental organic agriculture field stations. Three factors were evaluated: organic fertilizer application (with and without), row spacing (15 and 50 cm), and bacterial inoculation (H. diazotrophicus and control without bacteria). Hartmannibacter diazotrophicus was detected by quantitative polymerase chain reaction on the roots (up to 5 x 105 copies g-1 dry weight) until advanced developmental stages under field conditions during two seasons, and mostly in one farm. Correlation analysis showed a significant effect of H. diazotrophicus copy numbers on the yield parameters straw yield (increase of 453 kg ha-1 in wheat compared to the mean) and crude grain protein concentration (increase of 0.30% in wheat and 0.80% in barley compared to the mean). Our findings showed an apparently constant presence of H. diazotrophicus on both wheat and barley roots until 273 and 119 days after seeding, respectively, and its addition and concentration in the roots are associated with higher yields in one crop.

Hartmannibacter diazotrophicus used for seed inoculation of winter wheat and spring barely was able to colonize crop roots and affect yield parameters in a field experiment.

Harvard Citation style: Quiroga, S., Rosado-Porto, D., Ratering, S., Rekowski, A., Schulz, F., Krutych, M., et al. (2024) Long-term detection of Hartmannibacter diazotrophicus on winter wheat and spring barley roots under field conditions revealed positive correlations on yield parameters with the bacterium abundance, FEMS Microbiology Ecology, 100(3), Article fiae023. https://doi.org/10.1093/femsec/fiae023

APA Citation style: Quiroga, S., Rosado-Porto, D., Ratering, S., Rekowski, A., Schulz, F., Krutych, M., Zoerb, C., & Schnell, S. (2024). Long-term detection of Hartmannibacter diazotrophicus on winter wheat and spring barley roots under field conditions revealed positive correlations on yield parameters with the bacterium abundance. FEMS Microbiology Ecology. 100(3), Article fiae023. https://doi.org/10.1093/femsec/fiae023