Journal article
Authors list: Roxlau, Elsa T.; Pak, Oleg; Hadzic, Stefan; Garcia-Castro, Claudia F.; Gredic, Marija; Wu, Cheng-Yu; Schaeffer, Julia; Selvakumar, Balachandar; Pichl, Alexandra; Spiegelberg, David; Deutscher, Janik; Bednorz, Mariola; Schaefer, Katharina; Kraut, Simone; Kosanovic, Djuro; Zeidan, Esraa M.; Kojonazarov, Baktybek; Herold, Susanne; Strielkov, Ievgen; Guenther, Andreas; Wilhelm, Jochen; Khalifa, Mohamed M. A.; Taye, Ashraf; Brandes, Ralf P.; Hecker, Matthias; Grimminger, Friedrich; Ghofrani, Hossein A.; Schermuly, Ralph T.; Seeger, Werner; Sommer, Natascha; Weissmann, Norbert
Publication year: 2023
Journal: European Respiratory Journal
Volume number: 61
Issue number: 6
ISSN: 0903-1936
eISSN: 1399-3003
Open access status: Hybrid
DOI Link: https://doi.org/10.1183/13993003.00951-2022
Publisher: European Respiratory Society
Abstract:
Background Electronic cigarette (e-cigarette) vapour is gaining popularity as an alternative to tobacco smoking and can induce acute lung injury. However, the specific role of nicotine in e-cigarette vapour and its long-term effects on the airways, lung parenchyma and vasculature remain unclear.Results In vitro exposure to nicotine-containing e-cigarette vapour extract (ECVE) or to nicotine-free e-cigarette vapour extract (NF ECVE) induced changes in gene expression of epithelial cells and pulmonary arterial smooth muscle cells (PASMCs), but ECVE in particular caused functional alterations (e.g. a decrease in human and mouse PASMC proliferation by 29.3 & PLUSMN;5.3% and 44.3 & PLUSMN;8.4%, respectively). Additionally, acute inhalation of nicotine-containing e-cigarette vapour (ECV) but not nicotine-free e-cigarette vapour (NF ECV) increased pulmonary endothelial permeability in isolated lungs. Long-term in vivo exposure of mice to ECV for 8 months significantly increased the number of inflammatory cells, in particular lymphocytes, compared to control and NF ECV in the bronchoalveolar fluid (BALF) (ECV: 853.4 & PLUSMN;150.8 cells & BULL;mL-1; control: 37.0 & PLUSMN;21.1 cells & BULL;mL-1; NF ECV: 198.6 & PLUSMN;94.9 cells & BULL;mL-1) and in lung tissue (ECV: 25.7 & PLUSMN;3.3 cells & BULL;mm-3; control: 4.8 & PLUSMN;1.1 cells & BULL;mm-3; NF ECV: 14.1 & PLUSMN;2.2 cells & BULL;mm-3). BALF cytokines were predominantly increased by ECV. Moreover, ECV caused significant changes in lung structure and function (e.g. increase in airspace by 17.5 & PLUSMN;1.4% compared to control), similar to mild tobacco smoke-induced alterations, which also could be detected in the NF ECV group, albeit to a lesser degree. In contrast, the pulmonary vasculature was not significantly affected by ECV or NF ECV.Conclusions NF ECV components induce cell type-specific effects and mild pulmonary alterations, while inclusion of nicotine induces significant endothelial damage, inflammation and parenchymal alterations.
Citation Styles
Harvard Citation style: Roxlau, E., Pak, O., Hadzic, S., Garcia-Castro, C., Gredic, M., Wu, C., et al. (2023) Nicotine promotes e-cigarette vapour-induced lung inflammation and structural alterations, European Respiratory Journal, 61(6), Article 2200951. https://doi.org/10.1183/13993003.00951-2022
APA Citation style: Roxlau, E., Pak, O., Hadzic, S., Garcia-Castro, C., Gredic, M., Wu, C., Schaeffer, J., Selvakumar, B., Pichl, A., Spiegelberg, D., Deutscher, J., Bednorz, M., Schaefer, K., Kraut, S., Kosanovic, D., Zeidan, E., Kojonazarov, B., Herold, S., Strielkov, I., ...Weissmann, N. (2023). Nicotine promotes e-cigarette vapour-induced lung inflammation and structural alterations. European Respiratory Journal. 61(6), Article 2200951. https://doi.org/10.1183/13993003.00951-2022
Keywords
EMPHYSEMA; EXTRACTS
