Journal article

Publication year: 2023

Journal: Frontiers in Immunology

Volume number: 14

ISSN: 1664-3224

Open access status: Gold

DOI Link: https://doi.org/10.3389/fimmu.2023.1125667

Publisher: Frontiers Media

Abstract: 
Neutrophil extracellular traps (NET) formation is one important host innate defense mechanism elicited by polymorphonuclear neutrophils (PMN). NETs are composed by chromatin and proteins with microbicidal and signaling activity. So far, there is one report on Toxoplasma gondii-triggered NETs in cattle, however, exact mechanisms, including signalling pathways and dynamics governing this reaction remain largely unknown. Recently, involvement of cell cycle proteins was demonstrated for phorbol myristate acetate (PMA)-triggered human PMN-derived NETs. Here, we studied the involvement of cell cycle proteins in T. gondii-induced NETs in exposed bovine PMN. Through confocal and transmission electron microscopy we discovered that Ki-67 and lamin B1 signals are upregulated and relocated during T. gondii-induced NETosis. Nuclear membrane disruption was also observed as a hallmark of NET formation in bovine PMN confronted with viable T. gondii tachyzoites, mimicking some steps of mitosis. However, we did not observe centrosome duplication as previously described for human PMN-derived NET formation stimulated with PMA.

Harvard Citation style: Velasquez, Z., Peixoto, R., Gaertner, U., Hermosilla, C., Taubert, A. and Conejeros, I. (2023) Dynamics of cell cycle proteins involved in Toxoplasma gondii-induced bovine NET formation, Frontiers in Immunology, 14, Article 1125667. https://doi.org/10.3389/fimmu.2023.1125667

APA Citation style: Velasquez, Z., Peixoto, R., Gaertner, U., Hermosilla, C., Taubert, A., & Conejeros, I. (2023). Dynamics of cell cycle proteins involved in Toxoplasma gondii-induced bovine NET formation. Frontiers in Immunology. 14, Article 1125667. https://doi.org/10.3389/fimmu.2023.1125667