Journal article

Publication year: 2022

Journal: Small Ruminant Research

Volume number: 216

ISSN: 0921-4488

eISSN: 1879-0941

DOI Link: https://doi.org/10.1016/j.smallrumres.2022.106828

Publisher: Elsevier

Abstract: 
Six different marker sets with a total number of 19 microsatellite marker were studied in four different sheep breeds in Germany. The aim of this work was to develop a reliable and cost-effective tool for routine parentage testing in sheep. By calculating diversity parameters such as Na, AR, He, Ho and PIC, the validity of each marker was examined. In order to be able to make a statement about the certainty of paternal assignment for every marker set, Pe1 and cPe1 were determined. With cPe1 values above 0.999, all investigated marker sets showed a good reliability. For routine application in practice, a marker set consisting of eight microsatellite marker with a cPe1 of 0.9996 is suggested which fulfills the requirements with respect to reliability, work effort and costs.

Harvard Citation style: Ossowski, N., Wilkens, J., Mendel, C., Seichter, D., Russ, I., Wehrend, A., et al. (2022) Analysis of six microsatellite marker sets for parentage testing in four sheep breeds in Germany, Small Ruminant Research, 216, Article 106828. https://doi.org/10.1016/j.smallrumres.2022.106828

APA Citation style: Ossowski, N., Wilkens, J., Mendel, C., Seichter, D., Russ, I., Wehrend, A., & Thaller, G. (2022). Analysis of six microsatellite marker sets for parentage testing in four sheep breeds in Germany. Small Ruminant Research. 216, Article 106828. https://doi.org/10.1016/j.smallrumres.2022.106828