Journal article
Authors list: Xanthopoulou, Kyriaki; Imirzalioglu, Can; Walker, Sarah, V; Behnke, Michael; Dinkelacker, Ariane G.; Eisenbeis, Simone; Gastmeier, Petra; Goelz, Hanna; Kaeding, Nadja; Kern, Winfried, V; Kola, Axel; Kramme, Evelyn; Lucassen, Kai; Mischnik, Alexander; Peter, Silke; Rohde, Anna M.; Rupp, Jan; Tacconelli, Evelina; Tobys, David; Vehreschild, Maria J. G. T.; Wille, Julia; Seifert, Harald; Higgins, Paul G.
Publication year: 2022
Journal: Antibiotics
Volume number: 11
Issue number: 10
ISSN: 2079-6382
Open access status: Gold
DOI Link: https://doi.org/10.3390/antibiotics11101286
Publisher: MDPI
Abstract:
To analyse the epidemiology and population structure of third-generation cephalosporin-resistant (3GCR) and carbapenem-resistant (CR) Klebsiella pneumoniae complex isolates, patients were screened for rectal colonisation with 3GCR/CR K. pneumoniae complex on admission to six German university hospitals (2016-2019). Also collected were 3GCR/CR and susceptible K. pneumoniae isolates from patients with bloodstream infections (2016-2018). Whole-genome sequencing was performed followed by multilocus sequencing typing (MLST), core-genome MLST, and resistome and virulome analysis. The admission prevalence of 3GCR K. pneumoniae complex isolates during the 4-year study period was 0.8%, and 1.0 bloodstream infection per 1000 patient admissions was caused by K. pneumoniae complex (3GCR prevalence, 15.1%). A total of seven K. pneumoniae complex bloodstream isolates were CR (0.8%). The majority of colonising and bloodstream 3GCR isolates were identified as K. pneumoniae, 96.7% and 98.8%, respectively; the remainder were K. variicola and K. quasipneumoniae. cgMLST showed a polyclonal population of colonising and bloodstream isolates, which was also reflected by MLST and virulome analysis. CTX-M-15 was the most prevalent extended-spectrum beta-lactamase, and 29.7% of the colonising and 48.8% of the bloodstream isolates were high-risk clones. The present study provides an insight into the polyclonal 3GCR K. pneumoniae population in German hospitals.
Citation Styles
Harvard Citation style: Xanthopoulou, K., Imirzalioglu, C., Walker, S., Behnke, M., Dinkelacker, A., Eisenbeis, S., et al. (2022) Surveillance and Genomic Analysis of Third-Generation Cephalosporin-Resistant and Carbapenem-Resistant Klebsiella pneumoniae Complex in Germany, Antibiotics, 11(10), Article 1286. https://doi.org/10.3390/antibiotics11101286
APA Citation style: Xanthopoulou, K., Imirzalioglu, C., Walker, S., Behnke, M., Dinkelacker, A., Eisenbeis, S., Gastmeier, P., Goelz, H., Kaeding, N., Kern, W., Kola, A., Kramme, E., Lucassen, K., Mischnik, A., Peter, S., Rohde, A., Rupp, J., Tacconelli, E., Tobys, D., ...Higgins, P. (2022). Surveillance and Genomic Analysis of Third-Generation Cephalosporin-Resistant and Carbapenem-Resistant Klebsiella pneumoniae Complex in Germany. Antibiotics. 11(10), Article 1286. https://doi.org/10.3390/antibiotics11101286
Keywords
BETA-LACTAMASES; bloodstream infections; carbapenem resistance; colonisation; Klebsiella pneumoniae complex; third-generation cephalosporin resistance; typing; VARIICOLA
