Journal article
Authors list: Romero, Rossana; Zarzycka, Agnieszka; Preussner, Mathieu; Fischer, Florence; Hain, Torsten; Herrmann, Jan-Paul; Roth, Katrin; Keber, Corinna U.; Suryamohan, Kushal; Raifer, Hartmann; Luu, Maik; Leister, Hanna; Bertrams, Wilhelm; Klein, Matthias; Shams-Eldin, Hosam; Jacob, Ralf; Mollenkopf, Hans-Joachim; Rajalingam, Krishnaraj; Visekruna, Alexander; Steinhoff, Ulrich
Publication year: 2022
Journal: Microbiome
Volume number: 10
Issue number: 1
ISSN: 2049-2618
Open access status: Gold
DOI Link: https://doi.org/10.1186/s40168-022-01353-5
Publisher: BioMed Central
Background: The intestinal microbiota fundamentally guides the development of a normal intestinal physiology, the education, and functioning of the mucosal immune system. The Citrobacter rodentium-carrier model in germ-free (GF) mice is suitable to study the influence of selected microbes on an otherwise blunted immune response in the absence of intestinal commensals. Results: Here, we describe that colonization of adult carrier mice with 14 selected commensal microbes (OMM12 + MC2) was sufficient to reestablish the host immune response to enteric pathogens; this conversion was facilitated by maturation and activation of the intestinal blood vessel system and the step- and timewise stimulation of innate and adaptive immunity. While the immature colon of C. rodentium-infected GF mice did not allow sufficient extravasation of neutrophils into the gut lumen, colonization with OMM12 + MC2 commensals initiated the expansion and activation of the visceral vascular system enabling granulocyte transmigration into the gut lumen for effective pathogen elimination. Conclusions: Consortium modeling revealed that the addition of two facultative anaerobes to the OMM12 community was essential to further progress the intestinal development. Moreover, this study demonstrates the therapeutic value of a defined consortium to promote intestinal maturation and immunity even in adult organisms.
Abstract:
Citation Styles
Harvard Citation style: Romero, R., Zarzycka, A., Preussner, M., Fischer, F., Hain, T., Herrmann, J., et al. (2022) Selected commensals educate the intestinal vascular and immune system for immunocompetence, Microbiome, 10(1), Article 158. https://doi.org/10.1186/s40168-022-01353-5
APA Citation style: Romero, R., Zarzycka, A., Preussner, M., Fischer, F., Hain, T., Herrmann, J., Roth, K., Keber, C., Suryamohan, K., Raifer, H., Luu, M., Leister, H., Bertrams, W., Klein, M., Shams-Eldin, H., Jacob, R., Mollenkopf, H., Rajalingam, K., Visekruna, A., ...Steinhoff, U. (2022). Selected commensals educate the intestinal vascular and immune system for immunocompetence. Microbiome. 10(1), Article 158. https://doi.org/10.1186/s40168-022-01353-5
Keywords
Asymptomatic infection; Blood vessel development; CD146; CITROBACTER-RODENTIUM; COLONIZATION RESISTANCE; Commensal imprinting; C. rodentium; Enteric pathogen; Genome-guided microbiota; gut microbiota; Intestinal maturation; LINK; Microbial consortia; NEUTROPHILS; Oligo-Mouse-Microbiota; PANETH CELLS
