Journal article

Publication year: 2022

Journal: Bioresource Technology

Volume number: 358

ISSN: 0960-8524

eISSN: 1873-2976

Open access status: Hybrid

DOI Link: https://doi.org/10.1016/j.biortech.2022.127399

Publisher: Elsevier

Abstract: 
Enzyme combinations producing short-chain cello-oligosaccharides (COS) as major bio-products from cellulose of Miscanthus Mx2779 accessed through different pretreatment methods were compared. Over short hydrolysis times, processive endoglucanase TfCel9a produced a high percentage of cellotetraose and cellopentaose and is synergistic with endoglucanase CcCel9m for producing short oligomers from amorphous cellulose but had low activity on untreated Miscanthus. Hydrolysis of the latter improved when these were combined with a mutant cellobio/triohydrolase OsCelC7(- 105) and a lytic polysaccharide monooxygenase TrCel61a, a combination which also produced the highest COS yields from phosphoric acid swollen cellulose. Steam explosion pretreatment of Miscanthus increased COS yields, with/without phosphoric acid swelling, while increased swelling time (from 20 to 45 min) also increased yields but decreased the need for TrCel61a. The highest COS yields (933 mg/g glucan) and most stable product profile were obtained using ionic liquid [C2mim][OAc] pretreatment and the three enzyme mixture TfCel9a, Cel9m and OsCel7a(-105).

Harvard Citation style: Kendrick, E., Bhatia, R., Barbosa, F., Goldbeck, R., Gallagher, J. and Leak, D. (2022) Enzymatic generation of short chain cello-oligosaccharides from Miscanthus using different pretreatments, Bioresource Technology, 358, Article 127399. https://doi.org/10.1016/j.biortech.2022.127399

APA Citation style: Kendrick, E., Bhatia, R., Barbosa, F., Goldbeck, R., Gallagher, J., & Leak, D. (2022). Enzymatic generation of short chain cello-oligosaccharides from Miscanthus using different pretreatments. Bioresource Technology. 358, Article 127399. https://doi.org/10.1016/j.biortech.2022.127399