Journal article
Authors list: Zajonz, Thomas; Koch, Christian; Schwiddessen, Jan; Markmann, Melanie; Hecker, Matthias; Edinger, Fabian; Schmidt, Goetz; Boening, Andreas; Sander, Michael; Schneck, Emmanuel
Publication year: 2022
Journal: Journal of Clinical Medicine
Volume number: 11
Issue number: 11
eISSN: 2077-0383
Open access status: Gold
DOI Link: https://doi.org/10.3390/jcm11112994
Publisher: MDPI
Abstract:
The use of minimized extracorporeal circulation (MiECC) during cardiac surgery is associated with a reduced inflammatory reaction compared to conventional cardiopulmonary bypass (cCPB). Since it is unknown if MiECC also reduces the amount of free-circulating mitochondrial DNA (mtDNA), this study aims to compare MiECC-induced mtDNA release to that of cCPB as well as to identify potential relations between the plasma levels of mtDNA and an adverse outcome. Overall, 45 patients undergoing cardiac surgery with either cCPB or MiECC were included in the study. MtDNA encoding for NADH dehydrogenase 1 was quantified with quantitative polymerase chain reaction. The plasma amount of mtDNA was significantly lower in patients undergoing cardiac surgery with MiECC compared to cCPB (MiECC: 161.8 (65.5-501.9); cCPB 190.8 (82-705.7); p < 0.001). Plasma levels of mtDNA showed comparable kinetics independently of the study group and peaked during CPB (MiECC preoperative: 68.2 (26.5-104.9); MiECC 60 min after start of CPB: 536.5 (215.7-919.6); cCPB preoperative: 152.5 (80.9-207.6); cCPB 60 min after start of CPB: 1818.0 (844.2-3932.2); all p < 0.001). Patients offering an mtDNA blood concentration of >650 copies/mu L after the commencement of CPB had a 5-fold higher risk for postoperative atrial fibrillation independently of the type of cardiopulmonary bypass. An amount of mtDNA being higher than 650 copies/mu L showed moderate predictive power (AUROC 0.71 (0.53-071)) for the identification of postoperative atrial fibrillation. In conclusion, plasma levels of mtDNA were lower in patients undergoing cardiac surgery with MiECC compared to cCPB. The amount of mtDNA at the beginning of the CPB was associated with postoperative atrial fibrillation independent of the type of cardiopulmonary bypass.
Citation Styles
Harvard Citation style: Zajonz, T., Koch, C., Schwiddessen, J., Markmann, M., Hecker, M., Edinger, F., et al. (2022) Minimized Extracorporeal Circulation Is Associated with Reduced Plasma Levels of Free-Circulating Mitochondrial DNA Compared to Conventional Cardiopulmonary Bypass: A Secondary Analysis of an Exploratory, Prospective, Interventional Study, Journal of Clinical Medicine, 11(11), Article 2994. https://doi.org/10.3390/jcm11112994
APA Citation style: Zajonz, T., Koch, C., Schwiddessen, J., Markmann, M., Hecker, M., Edinger, F., Schmidt, G., Boening, A., Sander, M., & Schneck, E. (2022). Minimized Extracorporeal Circulation Is Associated with Reduced Plasma Levels of Free-Circulating Mitochondrial DNA Compared to Conventional Cardiopulmonary Bypass: A Secondary Analysis of an Exploratory, Prospective, Interventional Study. Journal of Clinical Medicine. 11(11), Article 2994. https://doi.org/10.3390/jcm11112994
Keywords
ATRIAL-FIBRILLATION; Cardiac surgery; CIRCUIT; coronary artery bypass graft; DAMPs; HEART-SURGERY; SIRS
