Journalartikel

Jahr der Veröffentlichung: 2022

Zeitschrift: Bioinformatics Advances

Bandnummer: 2

Heftnummer: 1

eISSN: 2635-0041

Open Access Status: Gold

DOI Link: https://doi.org/10.1093/bioadv/vbac009

Verlag: Oxford University Press

Abstract: 
In the era of next generation sequencing and beyond, the Sanger technique is still widely used for variant verification of inconclusive or ambiguous high-throughput sequencing results or as a low-cost molecular genetical analysis tool for single targets in many fields of study. Many analysis steps need time-consuming manual intervention. Therefore, we present here a pipeline-capable high-throughput solution with an optional Shiny web interface, that provides a binary mutation decision of hotspots together with plotted chromatograms including annotations via flat files.

Harvard-Zitierstil: Schmid, K., Dohmen, H., Ritschel, N., Selignow, C., Zohner, J., Sehring, J., et al. (2022) SangeR: the high-throughput Sanger sequencing analysis pipeline, Bioinformatics Advances, 2(1), Article vbac009. https://doi.org/10.1093/bioadv/vbac009

APA-Zitierstil: Schmid, K., Dohmen, H., Ritschel, N., Selignow, C., Zohner, J., Sehring, J., Acker, T., Amsel, D., & Stamatakis, A. (2022). SangeR: the high-throughput Sanger sequencing analysis pipeline. Bioinformatics Advances. 2(1), Article vbac009. https://doi.org/10.1093/bioadv/vbac009