Journalartikel
Autorenliste: Qi, Zenghua; Wang, Tao; Chen, Xiangmao; Wong, Chun Kit; Ding, Qianqian; Sauer, Heinrich; Chen, Zhi-Feng; Long, Cheng; Yao, Xiaoqiang; Cai, Zongwei; Tsang, Suk Ying
Jahr der Veröffentlichung: 2021
Zeitschrift: Frontiers in Molecular Biosciences
Bandnummer: 8
eISSN: 2296-889X
Open Access Status: Gold
DOI Link: https://doi.org/10.3389/fmolb.2021.699827
Verlag: Frontiers Media
Abstract:
Angiotensin II (Ang II) plays an important role in regulating various physiological processes. However, little is known about the existence of intracellular Ang II (iAng II), whether iAng II would regulate the automaticity of early differentiating cardiomyocytes, and the underlying mechanism involved. Here, iAng II was detected by immunocytochemistry and ultra-high performance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry in mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs) and neonatal rat ventricular myocytes. Expression of AT(1)R-YFP in mESC-CMs revealed that Ang II type 1 receptors were located on the surface membrane, while immunostaining of Ang II type 2 receptors (AT(2)R) revealed that AT(2)R were predominately located on the nucleus and the sarcoplasmic reticulum. While extracellular Ang II increased spontaneous action potentials (APs), dual patch clamping revealed that intracellular delivery of Ang II or AT(2)R activator C21 decreased spontaneous APs. Interestingly, iAng II was found to decrease the caffeine-induced increase in spontaneous APs and caffeine-induced calcium release, suggesting that iAng II decreased spontaneous APs via the AT(2)R- and ryanodine receptor-mediated pathways. This is the first study that provides evidence of the presence and function of iAng II in regulating the automaticity behavior of ESC-CMs and may therefore shed light on the role of iAng II in fate determination.
Zitierstile
Harvard-Zitierstil: Qi, Z., Wang, T., Chen, X., Wong, C., Ding, Q., Sauer, H., et al. (2021) Extracellular and Intracellular Angiotensin II Regulate the Automaticity of Developing Cardiomyocytes via Different Signaling Pathways, Frontiers in Molecular Biosciences, 8, Article 699827. https://doi.org/10.3389/fmolb.2021.699827
APA-Zitierstil: Qi, Z., Wang, T., Chen, X., Wong, C., Ding, Q., Sauer, H., Chen, Z., Long, C., Yao, X., Cai, Z., & Tsang, S. (2021). Extracellular and Intracellular Angiotensin II Regulate the Automaticity of Developing Cardiomyocytes via Different Signaling Pathways. Frontiers in Molecular Biosciences. 8, Article 699827. https://doi.org/10.3389/fmolb.2021.699827
Schlagwörter
angiotensin II receptor; developing cardiomyocytes; INTERNATIONAL UNION; spontaneous action potential; TRPC3
