Journalartikel
Autorenliste: Kruppa, Oscar Claudius; Gerlach, Doreen; Fan, Rong; Czermak, Peter
Jahr der Veröffentlichung: 2021
Seiten: 86-93
Zeitschrift: Electronic Journal of Biotechnology
Bandnummer: 54
ISSN: 0717-3458
Open Access Status: Gold
DOI Link: https://doi.org/10.1016/j.ejbt.2021.09.002
Verlag: Elsevier
Abstract:
Background: Planctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these species. We therefore developed a chemically defined medium for the model organism Planctopirus limnophila to increase biomass production. Results: We found that P. limnophila grows best with a 10 mM sodium phosphate buffer. The replacement of complex nitrogen sources with defined amino acid solutions did not inhibit growth. Screening for vitamin requirements revealed that only cyanocobalamin (B12) is needed for growth. We used response surface methodology to optimize the medium, resulting in concentrations of 10 g/L glucose, 34 mL/L Hutner's basal salts, 23.18 mM KNO3, 2.318 mM NH4Cl and 0.02 mg/L cyanocobalamin. The analysis of amino acid consumption allowed us to develop a customized amino acid solution lacking six of the amino acids present in Aminoplasmal 10%. Fed-batch cultivation in a bioreactor using the optimized medium achieved a final DOD600 of 46.8 +/- 0.5 after 108 h, corresponding to a cell dry weight of 13.6 +/- 0.7 g/L. Conclusions: The optimized chemically defined medium allowed us to produce larger amounts of biomass more quickly than reported in earlier studies. Further research should focus on triggering P. limnophila biofilm formation to activate the gene clusters responsible for secondary metabolism.
Zitierstile
Harvard-Zitierstil: Kruppa, O., Gerlach, D., Fan, R. and Czermak, P. (2021) Development of a chemically defined medium for Planctopirus limnophila to increase biomass production, Electronic Journal of Biotechnology, 54, pp. 86-93. https://doi.org/10.1016/j.ejbt.2021.09.002
APA-Zitierstil: Kruppa, O., Gerlach, D., Fan, R., & Czermak, P. (2021). Development of a chemically defined medium for Planctopirus limnophila to increase biomass production. Electronic Journal of Biotechnology. 54, 86-93. https://doi.org/10.1016/j.ejbt.2021.09.002
Schlagwörter
Amino acid analysis; Chemically defined medium; CHLAMYDIAE; Fed-batch cultivation; NOV.; PLANCTOMYCES-LIMNOPHILUS; Planctomycetes; Planctopirus limnophila; Response surface methodology; Vitamin screening