Journalartikel

3D holotomographic monitoring of Ca++ dynamics during ionophore-induced Neospora caninum tachyzoite egress from primary bovine host endothelial cells


AutorenlisteLarrazabal, C.; Hermosilla, C.; Taubert, A.; Conejeros, I

Jahr der Veröffentlichung2022

Seiten1169-1177

ZeitschriftParasitology Research

Bandnummer121

Heftnummer4

ISSN0932-0113

eISSN1432-1955

Open Access StatusHybrid

DOI Linkhttps://doi.org/10.1007/s00436-021-07260-2

VerlagSpringer


Abstract
Neospora caninum represents an obligate intracellular parasite that belongs to the phylum Apicomplexa and is a major abortive agent in bovines. During merogony, N. caninum tachyzoites invade and proliferate in host cells in vivo, including endothelial cells of lymphatic and blood vessels. The egress at the end of the lytic cycle is tightly regulated in apicomplexans. Evidence in Toxoplasma gondii shows that Ca++ signalling governs tachyzoite egress. Much less is known on egress mechanisms of N. caninum. Here, we show, using 3D live cell holotomographic microscopy in fluo-4 AM-loaded N. caninum-infected BUVEC, that treatments with the calcium ionophore A23187 at 24- and 42-h post-infection (h p. i.) induced a fast and sustained increase in Ca++ signals in parallel to tachyzoite egress. A23187 treatments exclusively triggered tachyzoite release at 42-h p. i. but failed to do so at 24-h p. i. indicating a role for meront maturation in calcium-induced tachyzoite egress. Overall, we show that live cell 3D holotomographic analysis in combination with epifluorescence is a suitable tool to study calcium dynamics related to coccidian egress or other important cell functions.



Zitierstile

Harvard-ZitierstilLarrazabal, C., Hermosilla, C., Taubert, A. and Conejeros, I. (2022) 3D holotomographic monitoring of Ca++ dynamics during ionophore-induced Neospora caninum tachyzoite egress from primary bovine host endothelial cells, Parasitology Research, 121(4), pp. 1169-1177. https://doi.org/10.1007/s00436-021-07260-2

APA-ZitierstilLarrazabal, C., Hermosilla, C., Taubert, A., & Conejeros, I. (2022). 3D holotomographic monitoring of Ca++ dynamics during ionophore-induced Neospora caninum tachyzoite egress from primary bovine host endothelial cells. Parasitology Research. 121(4), 1169-1177. https://doi.org/10.1007/s00436-021-07260-2



Schlagwörter


3D microscopyA23187ACIDOCALCISOMESEgressEXITFLUXHolotomographyINTRACELLULAR CALCIUMNeospora caninumTOXOPLASMA-GONDII


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