Journal article

Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa


Authors listGuzman, Juan; Sombolestani, Atena Sadat; Poehlein, Anja; Daniel, Rolf; Cleenwerck, Ilse; Vandamme, Peter; Vilcinskas, Andreas

Publication year2021

JournalInternational Journal of Systematic and Evolutionary Microbiology

Volume number71

Issue number3

ISSN1466-5026

eISSN1466-5034

DOI Linkhttps://doi.org/10.1099/ijsem.0.004666

PublisherMicrobiology Society


Abstract
A novel bacterium designated G55GP(T) and pertaining to the family Acetobacteraceae was isolated from the gut of the Madagascar hissing cockroach Gromphadorhina portentosa. The Gram-negative cells were rod-shaped and non-motile. The complete 16S rRNA sequence of the strain G55GP(T) showed the highest pairwise similarity to Gluconacetobacter johannae CFN-Cf-55(T) (95.35 %), suggesting it represents a potential new genus of the family Acetobacteraceae. Phylogenetic analysis based on 16S rRNA gene and 106 orthologous housekeeping protein sequences revealed that G55GPT forms a monophyletic clade with the genus Commensalibacter, which thus far has also been isolated exclusively from insects. The G55GP(T) genome size was 2.70 Mbp, and the G+C content was 45.4 mol%, which is lower than most acetic acid bacteria (51-68 mol%) but comparable to Swingsia samuiensis AH83(T) (45.1 mol%) and higher than Commensalibacter intestini A911(T) (36.8 mol%). Overall genome relatedness indices based on gene and protein sequences strongly supported the assignment of G55GP(T) to a new genus within the family Acetobacteraceae. The percentage of conserved proteins, which is a useful metric for genus differentiation, was below 54 % when comparing G55GP(T) to type strains of acetic acid bacteria, thus strongly supporting our hypothesis that G55GP(T) is a member of a yet-undescribed genus. The fatty acid composition of G55GP(T) differed from that of closely related acetic acid bacteria, particularly given the presence of C-19:1 omega 9c/omega 11c and the absence of C-14:0 and C-14:0 2-OH fatty acids. Strain G55GPT also differed in terms of metabolic features such as its ability to produce acid from d-mannitol, and its inability to produce acetic acid from ethanol or to oxidize glycerol to dihydroxyacetone. Based on the results of combined genomic, phenotypic and phylogenetic characterizations, isolate G55GP(T) (=LMG 31394(T)=DSM 111244(T)) is considered to represent a new species in a new genus, for which we propose the name Entomobacter blattae gen. nov., sp. nov.



Citation Styles

Harvard Citation styleGuzman, J., Sombolestani, A., Poehlein, A., Daniel, R., Cleenwerck, I., Vandamme, P., et al. (2021) Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa, International Journal of Systematic and Evolutionary Microbiology, 71(3), Article 004666. https://doi.org/10.1099/ijsem.0.004666

APA Citation styleGuzman, J., Sombolestani, A., Poehlein, A., Daniel, R., Cleenwerck, I., Vandamme, P., & Vilcinskas, A. (2021). Entomobacter blattae gen. nov., sp. nov., a new member of the Acetobacteraceae isolated from the gut of the cockroach Gromphadorhina portentosa. International Journal of Systematic and Evolutionary Microbiology. 71(3), Article 004666. https://doi.org/10.1099/ijsem.0.004666



Keywords


ACETIC-ACID BACTERIUMAcetobacteraceaecockroach microbiotaEntomobacter blattaeGromphadorhina portentosa


SDG Areas


Last updated on 2025-02-04 at 00:27