Journal article

Publication year: 2021

Journal: Plant Science: An international journal of experimental plant biology

Volume number: 307

ISSN: 0168-9452

eISSN: 1873-2259

DOI Link: https://doi.org/10.1016/j.plantsci.2021.110906

Publisher: Elsevier

Abstract: 
Plants secrete purple acid phosphatases (PAPs) under phosphorus (P) shortage but the contribution of plant PAPs to P acquisition is not well understood. The goals of this study were to investigate comprehensively the transcription patterns of PAPs under P shortage in poplar (Populus ? canescens), to identify secreted PAPs and to characterize their contribution to mobilize organic P. Phylogenetic analyses of the PAP family revealed 33 putative members. In this study, distinct, tissue-specific P responsive expression patterns could be shown for 23 PAPs in roots and leaves. Root-associated PAP activities were localized on the root surface by in-vivo staining. The activities of root-surface PAPs increased significantly under low P availability, but were suppressed by a PAP inhibitor and corresponded to elevated P uptake from ATP as an organic P source. By proteomic analyses of the root apoplast, we identified three newly secreted proteins under P shortage: PtPAP1 (Potri.005G233400) and two proteins with unknown functions (Potri.013G100800 and Potri.001G209300). Our results, based on the combination of transcriptome and proteome analyses with phosphatase activity assays, support that PtPAP1 plays a central role in enhanced P acquisition from organic sources, when the phosphate concentrations in soil are limited.

Harvard Citation style: Kavka, M., Majcherczyk, A., Kues, U. and Polle, A. (2021) Phylogeny, tissue-specific expression, and activities of root-secreted purple acid phosphatases for P uptake from ATP in P starved poplar, Plant Science: An international journal of experimental plant biology, 307, Article 110906. https://doi.org/10.1016/j.plantsci.2021.110906

APA Citation style: Kavka, M., Majcherczyk, A., Kues, U., & Polle, A. (2021). Phylogeny, tissue-specific expression, and activities of root-secreted purple acid phosphatases for P uptake from ATP in P starved poplar. Plant Science: An international journal of experimental plant biology. 307, Article 110906. https://doi.org/10.1016/j.plantsci.2021.110906