Journalartikel

Jahr der Veröffentlichung: 2021

Zeitschrift: Frontiers in Cell and Developmental Biology

Bandnummer: 9

ISSN: 2296-634X

Open Access Status: Gold

DOI Link: https://doi.org/10.3389/fcell.2021.645005

Verlag: Frontiers Media

Abstract: 
We aimed to investigate the molecular effect that adiponectin exerts on cementoblasts especially in the presence of compressive forces. OCCM-30 cells (M. Somerman, NIH, NIDCR, United States) were used. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and western blots were employed to verify if the mRNA and protein levels of adiponectin receptors (AdipoRs), mitogen-activated protein kinase (MAPK), and beta-catenin signaling were influenced by compressive forces or adiponectin. Moreover, siRNAs targeting P38 alpha, JNK1, ERK1, ERK2, and AdipoRs as well as pharmacological MAPK inhibition were performed. We found that compressive forces increase the expression of AdipoRs. Adiponectin and compression up-regulate P38 alpha,JNK1, ERK1, and ERK2 as well as beta-catenin gene expression. Western blots showed that co-stimuli activate the MAPK and beta-catenin signaling pathways. MAPK inhibition alters the compression-induced beta-catenin activation and the siRNAs targeting AdipoRs, P38 alpha, and JNK1, showing the interaction of single MAPK molecules and beta-catenin signaling in response to compression or adiponectin. Silencing by a dominantly negative version of P38 alpha and JNK1 attenuates adiponectin-induced TCF/LEF reporter activation. Together, we found that light compressive forces activate beta-catenin and MAPK signaling pathways. Adiponectin regulates beta-catenin signaling principally by inactivating the GSK-3 beta kinase activity. beta-Catenin expression was partially inhibited by MAPK blockade, indicating that MAPK plays a crucial role regulating beta-catenin during cementogenesis. Moreover, adiponectin modulates GSK-3 beta and beta-catenin mostly through AdipoR1. P38 alpha is a key connector between beta-catenin, TCF/LEF transcription, and MAPK signaling pathway.

Harvard-Zitierstil: Yong, J., von Bremen, J., Ruiz-Heiland, G. and Ruf, S. (2021) Adiponectin as Well as Compressive Forces Regulate in vitro β-Catenin Expression on Cementoblasts via Mitogen-Activated Protein Kinase Signaling Activation, Frontiers in Cell and Developmental Biology, 9, Article 645005. https://doi.org/10.3389/fcell.2021.645005

APA-Zitierstil: Yong, J., von Bremen, J., Ruiz-Heiland, G., & Ruf, S. (2021). Adiponectin as Well as Compressive Forces Regulate in vitro β-Catenin Expression on Cementoblasts via Mitogen-Activated Protein Kinase Signaling Activation. Frontiers in Cell and Developmental Biology. 9, Article 645005. https://doi.org/10.3389/fcell.2021.645005