Journalartikel
Autorenliste: Hussain, Ahmad; Grimm, Armin; Sheng, Wenjie; Zhang, Chaoyu; Al-Rawe, Marwah; Braeutigam, Karen; Abu Mraheil, Mobarak; Zeppernick, Felix; Meinhold-Heerlein, Ivo
Jahr der Veröffentlichung: 2021
Zeitschrift: Pharmaceuticals
Bandnummer: 14
Heftnummer: 4
eISSN: 1424-8247
Open Access Status: Gold
DOI Link: https://doi.org/10.3390/ph14040343
Verlag: MDPI
Abstract:
In the last few decades, antibody-based diagnostic and therapeutic applications have been well established in medicine and have revolutionized cancer managements by improving tumor detection and treatment. Antibodies are unique medical elements due to their powerful properties of being able to recognize specific antigens and their therapeutic mechanisms such as blocking specific pathways, antibody-dependent cellular cytotoxicity, and complement-dependent cytotoxicity. Furthermore, modification techniques have paved the way for improving antibody properties and to develop new classes of antibody-conjugate-based diagnostic and therapeutic agents. These techniques allow arming antibodies with various effector molecules. However, these techniques are utilizing the most frequently used amino acid residues for bioconjugation, such as cysteine and lysine. These bioconjugation approaches generate heterogeneous products with different functional and safety profiles. This is mainly due to the abundance of lysine and cysteine side chains. To overcome these limitations, different site-direct conjugation methods have been applied to arm the antibodies with therapeutic or diagnostics molecules to generate unified antibody conjugates with tailored properties. This review summarizes some of the enzyme-based site-specific conjugation approaches.
Zitierstile
Harvard-Zitierstil: Hussain, A., Grimm, A., Sheng, W., Zhang, C., Al-Rawe, M., Braeutigam, K., et al. (2021) Toward Homogenous Antibody Drug Conjugates Using Enzyme-Based Conjugation Approaches, Pharmaceuticals, 14(4), Article 343. https://doi.org/10.3390/ph14040343
APA-Zitierstil: Hussain, A., Grimm, A., Sheng, W., Zhang, C., Al-Rawe, M., Braeutigam, K., Abu Mraheil, M., Zeppernick, F., & Meinhold-Heerlein, I. (2021). Toward Homogenous Antibody Drug Conjugates Using Enzyme-Based Conjugation Approaches. Pharmaceuticals. 14(4), Article 343. https://doi.org/10.3390/ph14040343
Schlagwörter
antibody drug conjugate; enzyme-based conjugation; LIGATION; PEPTIDE TAG; SITE-SPECIFIC CONJUGATION; SORTASE; SURFACE-PROTEINS; VERSATILE
