Journalartikel

Jahr der Veröffentlichung: 2021

Zeitschrift: International Journal of Molecular Sciences

Bandnummer: 22

Heftnummer: 4

eISSN: 1422-0067

Open Access Status: Gold

DOI Link: https://doi.org/10.3390/ijms22041670

Verlag: MDPI

Abstract: 
CTRP-3 (C1q/TNF-related protein-3) is an adipokine with endocrine and immunological function. The impact of adipocyte CTRP-3 production on systemic CTRP-3 concentrations and on adipocyte biology is unknown. A murine model of adipocyte CTRP-3 knockout (KO) was established (via the Cre/loxP system). Serum adipokine levels were quantified by ELISA and adipose tissue (AT) gene expression by real-time PCR. Preadipocytes were isolated from AT and differentiated into adipocytes. Comparative transcriptome analysis was applied in adipocytes and liver tissue. Body weight and AT mass were reduced in CTRP-3 KO mice together with decreased serum leptin. In primary cells from visceral AT of KO mice, expression of adiponectin, progranulin, and resistin was induced, while peroxisome proliferator activated receptor gamma (PPAR gamma) was decreased. M1/M2 macrophage polarization markers were shifted to a more anti-inflammatory phenotype. CTRP-3 expression in AT did not contribute to serum concentrations. AT and liver morphology remained unaffected by CTRP-3 KO. Myelin transcription factor 1-like (Myt1l) was identified as a highly upregulated gene. In conclusion, adipocyte CTRP-3 has a role in adipogenesis and AT weight gain whereas adipocyte differentiation is not impaired by CTRP-3 deficiency. Since no effects on circulating CTRP-3 levels were observed, the impact of adipocyte CTRP-3 KO is limited to adipose tissue. Modified AT gene expression indicates a rather anti-inflammatory phenotype.

Harvard-Zitierstil: Schmid, A., Roderfeld, M., Gehl, J., Roeb, E., Nist, A., Chung, H., et al. (2021) C1q/TNF-Related Protein 3 (CTRP-3) Deficiency of Adipocytes Affects White Adipose Tissue Mass but Not Systemic CTRP-3 Concentrations, International Journal of Molecular Sciences, 22(4), Article 1670. https://doi.org/10.3390/ijms22041670

APA-Zitierstil: Schmid, A., Roderfeld, M., Gehl, J., Roeb, E., Nist, A., Chung, H., Stiewe, T., Karrasch, T., & Schaeffler, A. (2021). C1q/TNF-Related Protein 3 (CTRP-3) Deficiency of Adipocytes Affects White Adipose Tissue Mass but Not Systemic CTRP-3 Concentrations. International Journal of Molecular Sciences. 22(4), Article 1670. https://doi.org/10.3390/ijms22041670