Journalartikel

Jahr der Veröffentlichung: 2021

Zeitschrift: Science Advances

Bandnummer: 7

Heftnummer: 10

ISSN: 2375-2548

Open Access Status: Gold

DOI Link: https://doi.org/10.1126/sciadv.abf1004

Verlag: American Association for the Advancement of Science

Abstract: 
Coronaviruses infect many different species including humans. The last two decades have seen three zoonotic coronaviruses, with SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) causing a pandemic in 2020. Coronaviral non-structural proteins (nsps) form the replication-transcription complex (RTC). Nsp7 and nsp8 interact with and regulate the RNA-dependent RNA-polymerase and other enzymes in the RTC. However, the structural plasticity of nsp7+8 complexes has been under debate. Here, we present the framework of nsp7+8 complex stoichiometry and topology based on native mass spectrometry and complementary biophysical techniques of nsp7+8 complexes from seven coronaviruses in the genera Alpha- and Betacoronavirus including SARS-CoV-2. Their complexes cluster into three groups, which systematically form either heterotrimers or heterotetramers or both, exhibiting distinct topologies. Moreover, even at high protein concentrations, SARS-CoV-2 nsp7+8 consists primarily of heterotetramers. From these results, the different assembly paths can be pinpointed to specific residues and an assembly model proposed.

Harvard-Zitierstil: Krichel, B., Bylapudi, G., Schmidt, C., Blanchet, C., Schubert, R., Brings, L., et al. (2021) Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes, Science Advances, 7(10), Article eabf1004. https://doi.org/10.1126/sciadv.abf1004

APA-Zitierstil: Krichel, B., Bylapudi, G., Schmidt, C., Blanchet, C., Schubert, R., Brings, L., Koehler, M., Zenobi, R., Svergun, D., Lorenzen, K., Madhugiri, R., Ziebuhr, J., & Uetrecht, C. (2021). Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes. Science Advances. 7(10), Article eabf1004. https://doi.org/10.1126/sciadv.abf1004