Journalartikel

Jahr der Veröffentlichung: 2021

Seiten: 341-353

Zeitschrift: Parasitology

Bandnummer: 148

Heftnummer: 3

ISSN: 0031-1820

eISSN: 1469-8161

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1017/S0031182020002097

Verlag: Cambridge University Press

Abstract: 
Apicomplexan parasites are well-known to modulate their host cells at diverse functional levels. As such, apicomplexan-induced alteration of host cellular cell cycle was described and appeared dependent on both, parasite species and host cell type. As a striking evidence of species-specific reactions, we here show that Eimeria bovis drives primary bovine umbilical vein endothelial cells (BUVECs) into a senescence-like phenotype during merogony I. In line with senescence characteristics, E. bovis induces a phenotypic change in host cell nuclei being characterized by nucleolar fusion and heterochromatin-enriched peripheries. By fibrillarin staining we confirm nucleoli sizes to be increased and their number per nucleus to be reduced in E. bovis-infected BUVECs. Additionally, nuclei of E. bovis-infected BUVECs showed enhanced signals for HH3K9me2 as heterochromatin marker thereby indicating an infection-induced change in heterochromatin transition. Furthermore, E. bovis-infected BUVECs show an enhanced beta-galactosidase activity, which is a well-known marker of senescence. Referring to cell cycle progression, protein abundance profiles in E. bovis-infected endothelial cells revealed an up-regulation of cyclin E1 thereby indicating a cell cycle arrest at G(1)/S transition, signifying a senescence key feature. Similarly, abundance of G(2) phase-specific cyclin B1 was found to be downregulated at the late phase of macromeront formation. Overall, these data indicate that the slow proliferative intracellular parasite E. bovis drives its host endothelial cells in a senescence-like status. So far, it remains to be elucidated whether this phenomenon indeed reflects an intentionally induced mechanism to profit from host cell-derived energy and metabolites present in a non-dividing cellular status.

Harvard-Zitierstil: Velasquez, Z., Lopez-Osorio, S., Waiger, D., Manosalva, C., Pervizaj-Oruqaj, L., Herold, S., et al. (2021) Eimeria bovis infections induce G₁ cell cycle arrest and a senescence-like phenotype in endothelial host cells, Parasitology, 148(3), Article PII S0031182020002097. pp. 341-353. https://doi.org/10.1017/S0031182020002097

APA-Zitierstil: Velasquez, Z., Lopez-Osorio, S., Waiger, D., Manosalva, C., Pervizaj-Oruqaj, L., Herold, S., Hermosilla, C., & Taubert, A. (2021). Eimeria bovis infections induce G₁ cell cycle arrest and a senescence-like phenotype in endothelial host cells. Parasitology. 148(3), Article PII S0031182020002097, 341-353. https://doi.org/10.1017/S0031182020002097