Journal article

Publication year: 2020

Journal: Frontiers in Pharmacology

Volume number: 11

ISSN: 1663-9812

Open access status: Gold

DOI Link: https://doi.org/10.3389/fphar.2020.585346

Publisher: Frontiers Media

Abstract: 
Current clinical evidences suggest that circulating Adipokines such as Adiponectin can influence the ratio of orthodontic tooth movement. We aimed to investigate the effect that Adiponectin has on cementoblasts (OCCM-30) and on the intracellular signaling molecules of Mitogen-activated protein kinase (MAPK). We demonstrated that OCCM-30 cells express AdipoR1 and AdipoR2. Alizarin Red S staining revealed that Adiponectin increases mineralized nodule formation and quantitative AP activity in a dose-dependent manner. Adiponectin up-regulates the mRNA levels of AP, BSP, OCN, OPG, Runx-2 as well as F-Spondin. Adiponectin also increases the migration and proliferation of OCCM-30 cells. Moreover, Adiponectin induces a transient activation of JNK, P38, ERK1/2 and promotes the phosphorylation of STAT1 and STAT3. The activation of Adiponectin-mediated migration and proliferation was attenuated after pharmacological inhibition of P38, ERK1/2 and JNK in different degrees, whereas mineralization was facilitated by MAPK inhibition in varying degrees. Based on our results, Adiponectin favorably affect OCCM-30 cell migration, proliferation as well as cementogenesis. One of the underlying mechanisms is the activation of MAPK signaling pathway.

Harvard Citation style: Yong, J., von Bremen, J., Ruiz-Heiland, G. and Ruf, S. (2020) Adiponectin Interacts In-Vitro With Cementoblasts Influencing Cell Migration, Proliferation and Cementogenesis Partly Through the MAPK Signaling Pathway, Frontiers in Pharmacology, 11, Article 585346. https://doi.org/10.3389/fphar.2020.585346

APA Citation style: Yong, J., von Bremen, J., Ruiz-Heiland, G., & Ruf, S. (2020). Adiponectin Interacts In-Vitro With Cementoblasts Influencing Cell Migration, Proliferation and Cementogenesis Partly Through the MAPK Signaling Pathway. Frontiers in Pharmacology. 11, Article 585346. https://doi.org/10.3389/fphar.2020.585346