Journalartikel

Jahr der Veröffentlichung: 2020

Zeitschrift: Molecules

Bandnummer: 25

Heftnummer: 18

eISSN: 1420-3049

Open Access Status: Gold

DOI Link: https://doi.org/10.3390/molecules25184151

Verlag: MDPI

Abstract: 
Bone substitute materials loaded with mediators that stimulate fracture healing are demanded in the clinical treatment in trauma surgery and orthopedics. Brain-derived neurotrophic factor (BDNF) enhances the proliferation and differentiation of mesenchymal stem cells into osteoblast. To load the implants with BDNF, a drug delivery system that allows the release of BDNF under spatiotemporal control would improve functionality. Polyelectrolyte complex nanoparticles (PECNP) have been reported as a suitable drug delivery system. The suitability of PECNP in contact with osteocytes as the main cell type of bone is not known so far. Thus, we aimed to verify that BDNF and PECNP loaded with BDNF (PECNP+BDNF) as well as pure PECNP have no negative effects on osteocytes in vitro. Therefore, the murine osteocyte cell line MLO-Y4 was treated with BDNF and PECNP+BDNF. The effects on proliferation were analyzed by the BrdU test (n= 5). The results demonstrated a significant increase in proliferation 24 h after BDNF application, whereas PECNP+BDNF did not lead to significant changes. Thus, we conclude that BDNF is an appropriate mediator to stimulate osteocytes. Since the addition of PECNP did not affect the viability of osteocytes, we conclude that PECNP are a suitable drug delivery system for bone implants.

Harvard-Zitierstil: Loy, T., Vehlow, D., Kauschke, V., Mueller, M., Heiss, C. and Lips, K. (2020) Effects of BDNF and PEC Nanoparticles on Osteocytes, Molecules, 25(18), Article 4151. https://doi.org/10.3390/molecules25184151

APA-Zitierstil: Loy, T., Vehlow, D., Kauschke, V., Mueller, M., Heiss, C., & Lips, K. (2020). Effects of BDNF and PEC Nanoparticles on Osteocytes. Molecules. 25(18), Article 4151. https://doi.org/10.3390/molecules25184151