Journalartikel
Autorenliste: Bernardini, Chiara; Pagliari, Laura; De Rosa, Valeria; Almeida-Trapp, Marilia; Santi, Simonetta; Martini, Marta; Buoso, Sara; Loschi, Alberto; Loi, Nazia; Chiesa, Fiorella; Mithoefer, Axel; van Bel, Aart J. E.; Musetti, Rita
Jahr der Veröffentlichung: 2020
Zeitschrift: Scientific Reports
Bandnummer: 10
Heftnummer: 1
ISSN: 2045-2322
Open Access Status: Gold
DOI Link: https://doi.org/10.1038/s41598-020-71660-0
Verlag: Nature Research
Abstract:
The proteins AtSEOR1 and AtSEOR2 occur as conjugates in the form of filaments in sieve elements of Arabidopsis thaliana. A reduced phytoplasma titre found in infected defective-mutant Atseor1ko plants in previous work raised the speculation that non-conjugated SEOR2 is involved in the phytohormone-mediated suppression of Chrysanthemum Yellows (CY)-phytoplasma infection transmitted by Euscelidius variegatus (Ev). This early and long-lasting SEOR2 impact was revealed in Atseor1ko plants by the lack of detectable phytoplasmas at an early stage of infection (symptomless plants) and a lower phytoplasma titre at a later stage (fully symptomatic plants). The high insect survival rate on Atseor1ko line and the proof of phytoplasma infection at the end of the acquisition access period confirmed the high transmission efficiency of CY-phytoplasma by the vectors. Transmission electron microscopy analysis ruled out a direct role of SE filament proteins in physical phytoplasma containment. Time-correlated HPLC-MS/MS-based phytohormone analyses revealed increased jasmonate levels in midribs of Atseor1ko plants at an early stage of infection and appreciably enhanced levels of indole acetic acid and abscisic acid at the early and late stages. Effects of Ev-probing on phytohormone levels was not found. The results suggest that SEOR2 interferes with phytohormonal pathways in Arabidopsis midrib tissues in order to establish early defensive responses to phytoplasma infection.
Zitierstile
Harvard-Zitierstil: Bernardini, C., Pagliari, L., De Rosa, V., Almeida-Trapp, M., Santi, S., Martini, M., et al. (2020) Pre-symptomatic modified phytohormone profile is associated with lower phytoplasma titres in an Arabidopsis seor1ko line, Scientific Reports, 10(1), Article 14770. https://doi.org/10.1038/s41598-020-71660-0
APA-Zitierstil: Bernardini, C., Pagliari, L., De Rosa, V., Almeida-Trapp, M., Santi, S., Martini, M., Buoso, S., Loschi, A., Loi, N., Chiesa, F., Mithoefer, A., van Bel, A., & Musetti, R. (2020). Pre-symptomatic modified phytohormone profile is associated with lower phytoplasma titres in an Arabidopsis seor1ko line. Scientific Reports. 10(1), Article 14770. https://doi.org/10.1038/s41598-020-71660-0
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