Journal article

Publication year: 2020

Journal: Cells

Volume number: 9

Issue number: 8

eISSN: 2073-4409

Open access status: Gold

DOI Link: https://doi.org/10.3390/cells9081755

Publisher: MDPI

Abstract: 
Investigation of the molecular dynamics in lung cancer is crucial for the development of new treatment strategies. Fibroblast growth factor (FGF) 14 belongs to the FGF family, which might play a crucial role in cancer progression. We analyzed lung adenocarcinoma (LUAC) patients samples and found that FGF14 was downregulated, correlating with reduced survival and oncogenic mutation status. FGF14 overexpression in lung cancer cell lines resulted in decreased proliferation, colony formation, and migration, as well as increased expression of epithelial markers and a decreased expression of mesenchymal markers, indicating a mesenchymal to epithelial transition in vitro. We verified these findings using small interfering RNA against FGF14 and further confirmed the suppressive effect of FGF14 in aNOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/SzJimmunodeficient xenograft tumor model. Moreover, FGF14 overexpressing tumor cell RNA sequencing data suggests that genes affected by FGF14 were related to the extracellular matrix, playing a role in proliferation and migration. Notably, newly identified FGF14 target genes, adenosine deaminase RNA specific B1 (ADARB1), collagen and calcium-binding epidermal growth factor domain-containing protein 1 (CCBE1), alpha 1 chain of collagen XI (COL11A1), and mucin 16 (MUC16) expression was negatively correlated with overall survival whenFGF14was downregulated in LUAC. These findings led us to suggest that FGF14 regulates proliferation and migration in LUAC.

Harvard Citation style: Turkowski, K., Herzberg, F., Guenther, S., Brunn, D., Weigert, A., Meister, M., et al. (2020) Fibroblast Growth Factor-14 Acts as Tumor Suppressor in Lung Adenocarcinomas, Cells, 9(8), Article 1755. https://doi.org/10.3390/cells9081755

APA Citation style: Turkowski, K., Herzberg, F., Guenther, S., Brunn, D., Weigert, A., Meister, M., Muley, T., Kriegsmann, M., Schneider, M., Winter, H., Thomas, M., Grimminger, F., Seeger, W., Pullamsetti, S., & Savai, R. (2020). Fibroblast Growth Factor-14 Acts as Tumor Suppressor in Lung Adenocarcinomas. Cells. 9(8), Article 1755. https://doi.org/10.3390/cells9081755