Journalartikel

Jahr der Veröffentlichung: 2020

Zeitschrift: PLoS Pathogens

Bandnummer: 16

Heftnummer: 8

ISSN: 1553-7366

eISSN: 1553-7374

Open Access Status: Gold

DOI Link: https://doi.org/10.1371/journal.ppat.1008835

Verlag: Public Library of Science

Abstract: 

Author summary Disease in plants depends on the outcome of interaction between the pathogen and the host plant. Pathogens use effector proteins to aid colonization by suppression of the host immune system. Identification of effector targets can pinpoint key components of plant defence in addition to aiding our understanding of how pathogens manipulate their hosts. Here, we see a downy mildew effector mimicking a plant transcriptional repression motif and interacting with a transcriptional co-repressor in order to manipulate the plant immunity transcriptional response and susceptibility to both a biotrophic and a necrotrophic pathogen.

Hyaloperonospora arabidopsidis(Hpa) is an oomycete pathogen causing Arabidopsis downy mildew. Effector proteins secreted from the pathogen into the plant play key roles in promoting infection by suppressing plant immunity and manipulating the host to the pathogen's advantage. One class of oomycete effectors share a conserved 'RxLR' motif critical for their translocation into the host cell. Here we characterize the interaction between an RxLR effector, HaRxL21 (RxL21), and the Arabidopsis transcriptional co-repressor Topless (TPL). We establish that RxL21 and TPL interact via an EAR motif at the C-terminus of the effector, mimicking the host plant mechanism for recruiting TPL to sites of transcriptional repression. We show that this motif, and hence interaction with TPL, is necessary for the virulence function of the effector. Furthermore, we provide evidence that RxL21 uses the interaction with TPL, and its close relative TPL-related 1, to repress plant immunity and enhance host susceptibility to both biotrophic and necrotrophic pathogens.

Harvard-Zitierstil: Harvey, S., Kumari, P., Lapin, D., Griebel, T., Hickman, R., Guo, W., et al. (2020) Downy Mildew effector HaRxL21 interacts with the transcriptional repressor TOPLESS to promote pathogen susceptibility, PLoS Pathogens, 16(8), Article e1008835. https://doi.org/10.1371/journal.ppat.1008835

APA-Zitierstil: Harvey, S., Kumari, P., Lapin, D., Griebel, T., Hickman, R., Guo, W., Zhang, R., Parker, J., Beynon, J., Denby, K., & Steinbrenner, J. (2020). Downy Mildew effector HaRxL21 interacts with the transcriptional repressor TOPLESS to promote pathogen susceptibility. PLoS Pathogens. 16(8), Article e1008835. https://doi.org/10.1371/journal.ppat.1008835