Journal article

Publication year: 2020

Journal: Respiratory Research

Volume number: 21

Issue number: 1

eISSN: 1465-993X

Open access status: Gold

DOI Link: https://doi.org/10.1186/s12931-020-01394-0

Publisher: BioMed Central

Abstract: 
Background Obesity and pulmonary hypertension (PH) share common characteristics, such as augmented inflammation and oxidative stress. However, the exact role of obesity in the pathology of PH is largely uninvestigated. Therefore, we have hypothesized that in the context of obesity the gender difference may have influence on development of PH in animal models of this disease. Methods Animal experiments were conducted in monocrotaline (MCT) and chronic hypoxia (HOX) models of PH. Lean and obese Zucker rats or B6 mice of both genders were used for MCT or HOX models, respectively. Echocardiography, hemodynamic measurements, histology and immuno-histochemistry were performed to analyze various parameters, such as right ventricular function and hypertrophy, hemodynamics, pulmonary vascular remodeling and lung inflammation. Results Both lean and obese male and female Zucker rats developed PH after a single MCT injection. However, negligible differences were seen between lean and obese male rats in terms of PH severity at the end stage of disease. Conversely, a more prominent and severe PH was observed in obese female rats compared to their lean counterparts. In contrast, HOX induced PH in lean and obese, male and female mice did not show any apparent differences. Conclusion Gender influences PH severity in obese MCT-injected rats. It is also an important factor associated with altered inflammation. However, further research is necessary to investigate and reveal the underlying mechanisms.

Harvard Citation style: Neupane, B., Sydykov, A., Pradhan, K., Vroom, C., Herden, C., Karnati, S., et al. (2020) Influence of gender in monocrotaline and chronic hypoxia induced pulmonary hypertension in obese rats and mice, Respiratory Research, 21(1), Article 136. https://doi.org/10.1186/s12931-020-01394-0

APA Citation style: Neupane, B., Sydykov, A., Pradhan, K., Vroom, C., Herden, C., Karnati, S., Ghofrani, H., Avdeev, S., Erguen, S., Schermuly, R., & Kosanovic, D. (2020). Influence of gender in monocrotaline and chronic hypoxia induced pulmonary hypertension in obese rats and mice. Respiratory Research. 21(1), Article 136. https://doi.org/10.1186/s12931-020-01394-0