Journalartikel
Autorenliste: Giricz, Zoltan; Makkos, Andras; Schreckenberg, Rolf; Poeling, Jochen; Loerchner, Holger; Kiss, Krisztina; Bencsik, Peter; Braun, Thomas; Schulz, Rainer; Ferdinandy, Peter; Schlueter, Klaus-Dieter
Jahr der Veröffentlichung: 2020
Zeitschrift: International Journal of Molecular Sciences
Bandnummer: 21
Heftnummer: 9
eISSN: 1422-0067
Open Access Status: Gold
DOI Link: https://doi.org/10.3390/ijms21093359
Verlag: MDPI
Abstract:
Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated adult rat cardiomyocytes to cell culture conditions by initiating a process known as cell spreading. This process mimics central aspects of cardiac remodeling, as it occurs subsequent to myocardial infarction. However, expression of swiprosin-1 in cardiac tissue and its regulation in vivo has not yet been addressed. The expression of swiprosin-1 was analyzed in mice, rat, and pig hearts undergoing myocardial infarction or ischemia/reperfusion with or without cardiac protection by ischemic pre- and postconditioning. In mouse hearts, swiprosin-1 protein expression was increased after 4 and 7 days in myocardial infarct areas specifically in cardiomyocytes as verified by immunoblotting and histology. In rat hearts, swiprosin-1 mRNA expression was induced within 7 days after ischemia/reperfusion but this induction was abrogated by conditioning. As in cultured cardiomyocytes, the expression of swiprosin-1 was associated with a coinduction of arrestin-2, suggesting a common mechanism of regulation. Rno-miR-32-3p and rno-miR-34c-3p were associated with the regulation pattern of both molecules. Moreover, induction of swiprosin-1 and ssc-miR-34c was also confirmed in the infarct zone of pigs. In summary, our data show that up-regulation of swiprosin-1 appears in the postischemic heart during cardiac remodeling and repair in different species.
Zitierstile
Harvard-Zitierstil: Giricz, Z., Makkos, A., Schreckenberg, R., Poeling, J., Loerchner, H., Kiss, K., et al. (2020) Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning, International Journal of Molecular Sciences, 21(9), Article 3359. https://doi.org/10.3390/ijms21093359
APA-Zitierstil: Giricz, Z., Makkos, A., Schreckenberg, R., Poeling, J., Loerchner, H., Kiss, K., Bencsik, P., Braun, T., Schulz, R., Ferdinandy, P., & Schlueter, K. (2020). Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning. International Journal of Molecular Sciences. 21(9), Article 3359. https://doi.org/10.3390/ijms21093359
Schlagwörter
CARDIAC PROTECTION; Cardiac regeneration; CARDIOLOGY WORKING GROUP; CELLULAR BIOLOGY; EUROPEAN-SOCIETY; miR-34; POSITION PAPER
