Journalartikel

Jahr der Veröffentlichung: 2020

Zeitschrift: Journal of Clinical Medicine

Bandnummer: 9

Heftnummer: 1

eISSN: 2077-0383

Open Access Status: Gold

DOI Link: https://doi.org/10.3390/jcm9010174

Verlag: MDPI

Abstract: 
This proof-of-concept study aimed to evaluate a novel method of flow cytometry-based quantification of neutrophil extracellular traps (NETs) in septic shock patients and to identify possible interactions between the number of free-circulating NETs and alterations of the coagulatory system. Patients suffering from septic shock, a matched control group (CTRL), and patients suffering from systemic inflammation after cardiac (CABG) or major abdominal surgery (MAS) were enrolled in this prospective proof-of-concept study. Compared to the matched controls, free-circulating NETs were significantly elevated in septic shock and postsurgical patients (data are presented in median (IQR)); septic shock: (2.7 (1.9-3.9); CABG: 2.7 (2.1-3.7); MAS: 2.7 (2.1-3.9); CTRL: 1.6 (1-2); CTRL vs. septic shock: p = 0.001; CTRL vs. CABG: p < 0.001; CTRL vs. MAS: p < 0.001). NETs correlated positively with FIBTEM mean clot firmness (MCF) in septic shock patients (r = 0.37, p < 0.01) while they correlated negatively in surgical patients (CABG: r = -0.28, p < 0.01; MAS: r = -0.25, p = 0.03). Flow-cytometric quantification of NETs showed a significant increase in free-circulating NETs under inflammatory conditions. Furthermore, this study hints to an association of the number of NETs with hypercoagulation in septic shock patients and hypocoagulation in surgery-induced inflammation.

Harvard-Zitierstil: Schneck, E., Mallek, F., Schiederich, J., Kramer, E., Markmann, M., Hecker, M., et al. (2020) Flow Cytometry-Based Quantification of Neutrophil Extracellular Traps Shows an Association with Hypercoagulation in Septic Shock and Hypocoagulation in Postsurgical Systemic Inflammation-A Proof-of-Concept Study, Journal of Clinical Medicine, 9(1), Article 174. https://doi.org/10.3390/jcm9010174

APA-Zitierstil: Schneck, E., Mallek, F., Schiederich, J., Kramer, E., Markmann, M., Hecker, M., Sommer, N., Weissmann, N., Pak, O., Michel, G., Hecker, A., Padberg, W., Boening, A., Sander, M., & Koch, C. (2020). Flow Cytometry-Based Quantification of Neutrophil Extracellular Traps Shows an Association with Hypercoagulation in Septic Shock and Hypocoagulation in Postsurgical Systemic Inflammation-A Proof-of-Concept Study. Journal of Clinical Medicine. 9(1), Article 174. https://doi.org/10.3390/jcm9010174