Journal article

Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation


Authors listde Laffolie, Jan; Sheridan, Diana; Reinshagen, Konrad; Wessel, Lucas; Zimmermann, Christian; Stricker, Sebastian; Lerch, Markus M.; Weigel, Markus; Hain, Torsten; Domann, Eugen; Rudloff, Silvia; Nichols, Buford L.; Naim, Hassan Y.; Zimmer, Klaus-Peter

Publication year2020

Pages3983-3995

JournalThe FASEB Journal

Volume number34

Issue number3

ISSN0892-6638

eISSN1530-6860

Open access statusHybrid

DOI Linkhttps://doi.org/10.1096/fj.201901758RR

PublisherWiley


Abstract
Background and aims: Intestinal adaptation in short bowel syndrome (SBS) includes morphologic processes and functional mechanisms. This study investigated whether digestive enzyme expression in the duodenum and colon is upregulated in SBS patients. Method: Sucrase-isomaltase (SI), lactase-phlorizin hydrolase (LPH), and neutral Aminopeptidase N (ApN) were analyzed in duodenal and colonic biopsies from nine SBS patients in a late stage of adaptation as well as healthy and disease controls by immunoelectron microscopy (IEM), Western blots, and enzyme activities. Furthermore, proliferation rates and intestinal microbiota were analyzed in the mucosal specimen. Results: We found significantly increased amounts of SI, LPH, and ApN in colonocytes in most SBS patients with large variation and strongest effect for SI and ApN. Digestive enzyme expression was only partially elevated in duodenal enterocytes due to a low proliferation level measured by Ki-67 staining. Microbiome analysis revealed high amounts of Lactobacillus resp. low amounts of Proteobacteria in SBS patients with preservation of colon and ileocecal valve. Colonic expression was associated with a better clinical course in single cases. Conclusion: In SBS patients disaccharidases and peptidases can be upregulated in the colon. Stimulation of this colonic intestinalization process by drugs, nutrients, and pre- or probiotics might offer better therapeutic approaches.



Citation Styles

Harvard Citation stylede Laffolie, J., Sheridan, D., Reinshagen, K., Wessel, L., Zimmermann, C., Stricker, S., et al. (2020) Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation, The FASEB Journal, 34(3), pp. 3983-3995. https://doi.org/10.1096/fj.201901758RR

APA Citation stylede Laffolie, J., Sheridan, D., Reinshagen, K., Wessel, L., Zimmermann, C., Stricker, S., Lerch, M., Weigel, M., Hain, T., Domann, E., Rudloff, S., Nichols, B., Naim, H., & Zimmer, K. (2020). Digestive enzyme expression in the large intestine of children with short bowel syndrome in a late stage of adaptation. The FASEB Journal. 34(3), 3983-3995. https://doi.org/10.1096/fj.201901758RR



Keywords


ADULT PATIENTSENTERAL NUTRITIONMICROBIOTA SIGNATURESParenteral nutritionpediatric intestinal failurepediatric short bowel syndromePrognostic factorsRAT MODELRESECTION

Last updated on 2025-10-06 at 11:07