Journal article

Publication year: 2019

Journal: Biology

Volume number: 8

Issue number: 4

eISSN: 2079-7737

Open access status: Gold

DOI Link: https://doi.org/10.3390/biology8040078

Publisher: MDPI

Abstract: 
Besnoitia besnoiti tachyzoites infect and develop in bovine endothelial cells in vivo and trigger the release of neutrophil extracellular traps (NETs) from bovine polymorphonuclear neutrophils (PMN). The purpose of this study was to analyze if pure B. besnoiti tachyzoite-triggered NETs would damage endothelial host cells and subsequently influence intracellular development and proliferation of B. besnoiti tachyzoites in primary bovine endothelial cells. For comparison purposes, isolated A23187-induced NETs were also used. Thus, we here evaluated endothelial host cell damage triggered by histone 2A (H2A) and B. besnoiti tachyzoite-induced NET preparations and furthermore estimated the effects of PMN floating over B. besnoiti-infected endothelium under physiological flow conditions on endothelial host cell viability. Overall, all treatments (H2A, B. besnoiti-triggered NETs and floating PMN) induced endothelial cell death of B. besnoiti-infected host cells. However, though host cell damage led to significantly altered intracellular parasite development with respect to parasitophorous vacuole diameter and numbers, the total proliferation of the parasite over time was not significantly affected by these treatments thereby denying any direct effect of NETs on intracellular B. besnoiti replication.

Harvard Citation style: Conejeros, I., Velasquez, Z., Grob, D., Zhou, E., Salecker, H., Hermosilla, C., et al. (2019) Histone H2A and Bovine Neutrophil Extracellular Traps Induce Damage of Besnoitia besnoiti-Infected Host Endothelial Cells but Fail to Affect Total Parasite Proliferation, Biology, 8(4), Article 78. https://doi.org/10.3390/biology8040078

APA Citation style: Conejeros, I., Velasquez, Z., Grob, D., Zhou, E., Salecker, H., Hermosilla, C., & Taubert, A. (2019). Histone H2A and Bovine Neutrophil Extracellular Traps Induce Damage of Besnoitia besnoiti-Infected Host Endothelial Cells but Fail to Affect Total Parasite Proliferation. Biology. 8(4), Article 78. https://doi.org/10.3390/biology8040078