Journal article
Authors list: Tighe, Robert M.; Redente, Elizabeth F.; Yu, Yen-Rei; Herold, Susanne; Sperling, Anne, I; Curtis, Jeffrey L.; Duggan, Ryan; Swaminathan, Suchitra; Nakano, Hideki; Zacharias, William J.; Janssen, William J.; Freeman, Christine M.; Brinkman, Ryan R.; Singer, Benjamin D.; Jakubzick, Claudia, V; Misharin, Alexander, V
Publication year: 2019
Pages: 150-161
Journal: American Journal of Respiratory Cell and Molecular Biology
Volume number: 61
Issue number: 2
ISSN: 1044-1549
eISSN: 1535-4989
Open access status: Green
DOI Link: https://doi.org/10.1165/rcmb.2019-0191ST
Publisher: American Thoracic Society
Abstract:
Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
Citation Styles
Harvard Citation style: Tighe, R., Redente, E., Yu, Y., Herold, S., Sperling, A., Curtis, J., et al. (2019) Improving the Quality and Reproducibility of Flow Cytometry in the Lung An Official American Thoracic Society Workshop Report, American Journal of Respiratory Cell and Molecular Biology, 61(2), pp. 150-161. https://doi.org/10.1165/rcmb.2019-0191ST
APA Citation style: Tighe, R., Redente, E., Yu, Y., Herold, S., Sperling, A., Curtis, J., Duggan, R., Swaminathan, S., Nakano, H., Zacharias, W., Janssen, W., Freeman, C., Brinkman, R., Singer, B., Jakubzick, C., & Misharin, A. (2019). Improving the Quality and Reproducibility of Flow Cytometry in the Lung An Official American Thoracic Society Workshop Report. American Journal of Respiratory Cell and Molecular Biology. 61(2), 150-161. https://doi.org/10.1165/rcmb.2019-0191ST
Keywords
AUTOFLUORESCENCE; FACTOR RECEPTOR-ALPHA; flow cytometry; lung biology; MEMORY T-CELLS; MONONUCLEAR PHAGOCYTES; MURINE LUNG; REPRODUCIBILITY; SUBPOPULATIONS; SUBSETS
