Journal article
Authors list: Clausen, Frederik Banch; Barrett, Angela Natalie; Akkok, Cigdem Akalin; Armstrong-Fisher, Sylvia; Bergstrom, Karolina Danielsson; Boggione, Carolina Trucco; Baevre, Mette Silihagen; Choolani, Mahesh; Christiansen, Mette; Cotorruelo, Carlos; Drnovsek, Tadeja Dovc; Finning, Kirstin; Guz, Katarzyna; de Haas, Masja; Haimila, Katri; Halldorsdottir, Anna Margret; Hellberg, Asa; Henny, Christine; Holmertz, Camilla; Al Houghton, Jayne; Hyland, Catherine; Jakobsen, Marianne Antonius; Kvitland, Mona Andersen; Lambert, Mark; Legler, Tobias J.; Liew, Yew-Wah; Muniz-Diaz, Eduardo; Mortberg, Anette; Niederhauser, Christoph; Nogues, Nuria; Nystrom, Sofia; Olsson, Martin L.; Orzinska, Agnieszka; Parks, Michael; Rietkotter, Eva; Ryan, Helen; Sachs, Ulrich J.; van der Schoot, Ellen; Silcock, Lee; Steffensen, Rudi; Sulin, Kati; Sorensen, Anne Solling; Tarrant, Sarah; Thorlacius, Steinunn; Wienzek-Lischka, Sandra; Wikman, Agneta; Wulf-Johansson, Helle; Zupan, Mojca; Dziegiel, Morten Hanefeld
Publication year: 2019
Pages: 386-393
Journal: Vox Sanguinis
Volume number: 114
Issue number: 4
ISSN: 0042-9007
eISSN: 1423-0410
Open access status: Green
DOI Link: https://doi.org/10.1111/vox.12768
Publisher: Wiley
Abstract:
Background and Objectives Fetal RHD genotyping of cell-free fetal DNA from RhD-negative pregnant women can be used to guide targeted antenatal and postnatal anti-D prophylaxis for the prevention of RhD immunization. To assure the quality of clinical testing, we conducted an external quality assessment workshop with the participation of 28 laboratories. Materials and Methods Aliquots of pooled maternal plasma were sent to each laboratory. One sample was positive, and the second sample was negative for fetal RHD, verified by pre-workshop testing using quantitative real-time PCR (qPCR) analysis of RHD exons 4, 5, 7 and 10. Plasma samples were shipped at room temperature. A reporting scheme was supplied for data collection, including questions regarding the methodological setup, results and clinical recommendations. Different methodological approaches were used, all employing qPCR with a total of eight different combinations of RHD exon targets. The samples were tested blindly. Results Fetal RHD genotyping was performed with no false-negative and no false-positive results. One inconclusive result was reported for the RHD-positive sample, and four inconclusive results were reported for the RHD-negative sample. All clinical conclusions were satisfactory. Conclusion This external quality assessment workshop demonstrates that despite the different approaches taken to perform the clinical assays, fetal RHD genotyping is a reliable laboratory assay to guide targeted use of Rh prophylaxis in a clinical setting.
Citation Styles
Harvard Citation style: Clausen, F., Barrett, A., Akkok, C., Armstrong-Fisher, S., Bergstrom, K., Boggione, C., et al. (2019) Noninvasive fetal RHD genotyping to guide targeted anti-D prophylaxis-an external quality assessment workshop, Vox Sanguinis, 114(4), pp. 386-393. https://doi.org/10.1111/vox.12768
APA Citation style: Clausen, F., Barrett, A., Akkok, C., Armstrong-Fisher, S., Bergstrom, K., Boggione, C., Baevre, M., Choolani, M., Christiansen, M., Cotorruelo, C., Drnovsek, T., Finning, K., Guz, K., de Haas, M., Haimila, K., Halldorsdottir, A., Hellberg, A., Henny, C., Holmertz, C., ...Dziegiel, M. (2019). Noninvasive fetal RHD genotyping to guide targeted anti-D prophylaxis-an external quality assessment workshop. Vox Sanguinis. 114(4), 386-393. https://doi.org/10.1111/vox.12768
Keywords
cell-free fetal DNA; external quality assessment; fetal RHD genotyping; PREGNANT-WOMEN; Rh prophylaxis; SANGUINIS INTERNATIONAL FORUM
