Journal article

Publication year: 2019

Pages: 459-475

Journal: Plant Systematics and Evolution

Volume number: 305

Issue number: 6

ISSN: 0378-2697

eISSN: 1615-6110

DOI Link: https://doi.org/10.1007/s00606-019-01585-3

Publisher: Springer

Abstract: 
We applied fluorescent in situ hybridization (FISH) using (GAA)(10) microsatellite repeat alone or in combination with oligo pSc119.2-1, pTa535-1, pAs1-1, 5S and 45S rDNA probes to identify individual chromosomes and produce banded karyotypes for five allopolyploid Aegilops species that were thought to possess M subgenome. Labelling patterns of GAA repeats allow identification of individual chromosomes in the studied species including diploid Ae. comosa subsp. heldreichii (2n=(MMh)-M-h) and Ae. umbellulata (2n=UU); and allopolyploid Ae. biuncialis (2n=(UUMMb)-U-b-M-b-M-b), Ae. geniculata (2n=(UUMMg)-U-g-M-g-M-g), Ae. columnaris (2n=(UUXXc)-U-c-X-c-X-c), Ae. neglecta (2n=(UUXXn)-U-n-X-n-X-n) and Ae. crassa (2n=4x=(DDXXcr)-D-1-X-1-X-cr and 2n=6x=(DDXXDDcr)-D-1-X-1-X-cr-D-cr-D-cr). Intraspecific polymorphisms of GAA labelling patterns were observed between different genotypes in each species. FISH results showed that the X* subgenomes of Ae. columnaris and Ae. neglecta are highly similar, implying that both species originated from a common ancestor. However, these X* subgenomes were also distinct from and more heterochromatic when compared to the M* subgenomes of Ae. biuncialis and Ae. geniculata. This suggests that the X* subgenomes may not have originated from the Ae. comosa, as previously thought. Although (GAA)(n) bands are relatively few and weak in Ae. tauschii (DD genome), all 4x Ae. crassa ((DDXXcr)-D-1-X-1-X-cr genome) chromosomes showed distinct GAA patterns. (GAA)(10) probe alone was not sufficient for the discrimination of all the chromosomes of 6x Ae. crassa ((DDXXDDcr)-D-1-X-1-X-cr-D-cr-D-cr), which needs a combination of (GAA)(10) with the pTa535-1 or pAs1-1 probes.

Harvard Citation style: Abdolmalaki, Z., Mirzaghaderi, G., Mason, A. and Badaeva, E. (2019) Molecular cytogenetic analysis reveals evolutionary relationships between polyploid Aegilops species, Plant Systematics and Evolution, 305(6), pp. 459-475. https://doi.org/10.1007/s00606-019-01585-3

APA Citation style: Abdolmalaki, Z., Mirzaghaderi, G., Mason, A., & Badaeva, E. (2019). Molecular cytogenetic analysis reveals evolutionary relationships between polyploid Aegilops species. Plant Systematics and Evolution. 305(6), 459-475. https://doi.org/10.1007/s00606-019-01585-3