Journalartikel
Autorenliste: Zhang, Ruiyan; Zhang, Ning; Mohri, Marzieh; Wu, Lisha; Eckert, Thomas; Krylov, Vadim B.; Antosova, Andrea; Ponikova, Slavomira; Bednarikova, Zuzana; Markart, Philipp; Guenther, Andreas; Norden, Bengt; Billeter, Martin; Schauer, Roland; Scheidig, Axel J.; Ratha, Bhisma N.; Bhunia, Anirban; Hesse, Karsten; Enani, Mushira Abdelaziz; Steinmeyer, Juergen; Petridis, Athanasios K.; Kozar, Tibor; Gazova, Zuzana; Nifantiev, Nikolay E.; Siebert, Hans-Christian
Jahr der Veröffentlichung: 2019
Seiten: 4206-4220
Zeitschrift: ACS Omega
Bandnummer: 4
Heftnummer: 2
ISSN: 2470-1343
Open Access Status: Gold
DOI Link: https://doi.org/10.1021/acsomega.8b02471
Verlag: American Chemical Society
Abstract:
Insulin and lysozyme share the common features of being prone to aggregate and having biomedical importance. Encapsulating lysozyme and insulin in micellar nanoparticles probably would prevent aggregation and facilitate oral drug delivery. Despite the vivid structural knowledge of lysozyme and insulin, the environment-dependent oligomerization (dimer, trimer, and multimer) and associated structural dynamics remain elusive. The knowledge of the intra- and intermolecular interaction profiles has cardinal importance for the design of encapsulation protocols. We have employed various biophysical methods such as NMR spectroscopy, X-ray crystallography, Thioflavin T fluorescence, and atomic force microscopy in conjugation with molecular modeling to improve the understanding of interaction dynamics during homo-oligomerization of lysozyme (human and hen egg) and insulin (porcine, human, and glargine). The results obtained depict the atomistic intra- and intermolecular interaction details of the homo-oligomerization and confirm the propensity to form fibrils. Taken together, the data accumulated and knowledge gained will further facilitate nanoparticle design and production with insulin or lysozyme-related protein encapsulation.
Zitierstile
Harvard-Zitierstil: Zhang, R., Zhang, N., Mohri, M., Wu, L., Eckert, T., Krylov, V., et al. (2019) Nanomedical Relevance of the Intermolecular Interaction Dynamics-Examples from Lysozymes and Insulins, ACS Omega, 4(2), pp. 4206-4220. https://doi.org/10.1021/acsomega.8b02471
APA-Zitierstil: Zhang, R., Zhang, N., Mohri, M., Wu, L., Eckert, T., Krylov, V., Antosova, A., Ponikova, S., Bednarikova, Z., Markart, P., Guenther, A., Norden, B., Billeter, M., Schauer, R., Scheidig, A., Ratha, B., Bhunia, A., Hesse, K., Enani, M., ...Siebert, H. (2019). Nanomedical Relevance of the Intermolecular Interaction Dynamics-Examples from Lysozymes and Insulins. ACS Omega. 4(2), 4206-4220. https://doi.org/10.1021/acsomega.8b02471
Schlagwörter
AMYLOID AGGREGATION; COARSE-GRAINED MODEL; MOLECULAR-DYNAMICS
