Journalartikel
Autorenliste: Doijad, Swapnil P.; Poharkar, Krupali, V; Kale, Satyajit B.; Kerkar, Savita; Kalorey, Dewanand R.; Kurkure, Nitin, V; Rawool, Deepak B.; Malik, Satya Veer Singh; Ahmad, Rafed Yassin; Hudel, Martina; Chaudhari, Sandeep P.; Abt, Birte; Overmann, Joerg; Weigel, Markus; Hain, Torsten; Barbuddhe, Sukhadeo B.; Chakraborty, Trinad
Jahr der Veröffentlichung: 2018
Seiten: 3285-3291
Zeitschrift: International Journal of Systematic and Evolutionary Microbiology
Bandnummer: 68
Heftnummer: 10
ISSN: 1466-5026
eISSN: 1466-5034
Open Access Status: Bronze
DOI Link: https://doi.org/10.1099/ijsem.0.002980
Verlag: Microbiology Society
Abstract:
Two Listeria-like isolates obtained from mangrove swamps in Goa, India were characterized using polyphasic combinations of phenotypic, chemotaxonomic and whole-genome sequence (WGS)-based approaches. The isolates presented as short, non-spore-forming, Gram-positive rods, that were non-motile, oxidase-negative, catalase-positive and exhibited a-haemolysis on 5 % sheep- and horse-blood agar plates. The 16S rRNA gene sequences exhibited 93.7-99.7 % nucleotide identity to other Listeria species and had less than 92 % nucleotide identity to species of closely related genera, indicating that the isolates are de facto members of the genus Listeria. Their overall fatty acid composition resembled that of other Listeria species, with quantitative differences in iso C-15(:0), anteiso C-15:0, iso C-16(:0), C-16(:0), iso C-17(:0) and anteiso C-17:0 fatty acid profiles. Phylogeny based on 406 core coding DNA sequences grouped these two isolates in a monophyletic Glade within the genus Listeria. WGS-based average nucleotide identity and in silico DNA-DNA hybridization values were lower than the recommended cutoff values of 95 and 70 %, respectively, to the other Listeria species, indicating that they are founding members of a novel Listeria species. We suggest the name Listeria goaensis sp. nov. be created and the type strain is ILCC801(T) (=KCTC 33909; =DSM 29886;=MCC 3285).
Zitierstile
Harvard-Zitierstil: Doijad, S., Poharkar, K., Kale, S., Kerkar, S., Kalorey, D., Kurkure, N., et al. (2018) Listeria goaensis sp nov., International Journal of Systematic and Evolutionary Microbiology, 68(10), pp. 3285-3291. https://doi.org/10.1099/ijsem.0.002980
APA-Zitierstil: Doijad, S., Poharkar, K., Kale, S., Kerkar, S., Kalorey, D., Kurkure, N., Rawool, D., Malik, S., Ahmad, R., Hudel, M., Chaudhari, S., Abt, B., Overmann, J., Weigel, M., Hain, T., Barbuddhe, S., & Chakraborty, T. (2018). Listeria goaensis sp nov.. International Journal of Systematic and Evolutionary Microbiology. 68(10), 3285-3291. https://doi.org/10.1099/ijsem.0.002980
Schlagwörter
Goa; haemolysis; L. goaensis; new taxa; STANDARD
