Journal article

Publication year: 2018

Pages: 462-470

Journal: Journal of infection in developing countries

Volume number: 12

Issue number: 6

ISSN: 1972-2680

Open access status: Gold

DOI Link: https://doi.org/10.3855/jidc.9862

Publisher: Journal of Infection in Developing Countries

Abstract: 

Introduction: Resistance to ciprofloxacin and tetracycline is increasing in the food chain especially in E. coli strains and more worrisome will be occurrence of extended-spectrum beta-lactamase (ESBL) producers among ciprofloxacin-and tetracycline-resistant isolates. This study was undertaken to investigate the occurrence and mechanism of ciprofloxacin-, tetracycline-and ESBL-resistant bacteria in poultry in Ibadan, Nigeria.

Methodology: Bacteria were isolated from poultry feces in two farms in Ibadan and identified by MALDI-TOF. Antibiotic susceptibility patterns of the isolates were determined by disc diffusion and Minimum Inhibitory Concentration (MIC) using Vitek-2 apparatus. Four tetracycline genes and six plasmids mediated quinolone resistance genes (PMQR) were investigated by PCR. Whole genome sequencing was done for strains that were ESBL producers.

Results: Bacterial strains (>= 10(5) cfu/mL) were counted on ciprofloxacin and tetracycline supplemented plates. 106 bacteria from 14 different species were identified with high resistance to quinolones, tetracycline and trimethoprim. 49% of the strains were E. coli with 90% resistance for nalidixic acid, moxifloxacin (94%), ciprofloxacin (88%) levofloxacin (78%) and tetracycline (77%). The genes tetA, tetB, qnrB, qnrS and qepA were detected with 37%, 4%, 35%, 4% and 2% prevalence in E. coli respectively. Three ESBL-producing E. coli of the sequence type ST-6359 were found and harboured blaCTX-M-15 located in the chromosome, at the same insertion site. All the ESBL producers harboured mutations in gyrA (S83L/D87N/D678E) and parC (S80I).

Conclusion: The observed high quinolones and tetracycline resistance with ESBL producers in this study calls for caution in the use of these antibiotics in poultry feeds.

Harvard Citation style: Ayandiran, T., Falgenhauer, L., Schmiede, J., Chakraborty, T. and Ayeni, F. (2018) High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria, Journal of infection in developing countries, 12(6), pp. 462-470. https://doi.org/10.3855/jidc.9862

APA Citation style: Ayandiran, T., Falgenhauer, L., Schmiede, J., Chakraborty, T., & Ayeni, F. (2018). High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria. Journal of infection in developing countries. 12(6), 462-470. https://doi.org/10.3855/jidc.9862