Journal article

Computational segmentation of collagen fibers in bone matrix indicates bone quality in ovariectomized rat spine


Authors listDaghma, Diaa Eldin S.; Malhan, Deeksha; Simon, Paul; Stoetzel, Sabine; Kern, Stefanie; Hassan, Fathi; Lips, Katrin Susanne; Heiss, Christian; El Khassawna, Thaqif

Publication year2018

Pages297-306

JournalJournal of Bone and Mineral Metabolism

Volume number36

Issue number3

ISSN0914-8779

eISSN1435-5604

DOI Linkhttps://doi.org/10.1007/s00774-017-0844-5

PublisherSpringer


Abstract
Bone loss varies according to disease and age and these variations affect bone cells and extracellular matrix. Osteoporosis rat models are widely investigated to assess mechanical and structural properties of bone; however, bone matrix proteins and their discrepant regulation of diseased and aged bone are often overlooked. The current study considered the spine matrix properties of ovariectomized rats (OVX) against control rats (Sham) at 16 months of age. Diseased bone showed less compact structure with inhomogeneous distribution of type 1 collagen (Col1) and changes in osteocyte morphology. Intriguingly, demineralization patches were noticed in the vicinity of blood vessels in the OVX spine. The organic matrix structure was investigated using computational segmentation of collagen fibril properties. In contrast to the aged bone, diseased bone showed longer fibrils and smaller orientation angles. The study shows the potential of quantifying transmission electron microscopy images to predict the mechanical properties of bone tissue.



Citation Styles

Harvard Citation styleDaghma, D., Malhan, D., Simon, P., Stoetzel, S., Kern, S., Hassan, F., et al. (2018) Computational segmentation of collagen fibers in bone matrix indicates bone quality in ovariectomized rat spine, Journal of Bone and Mineral Metabolism, 36(3), pp. 297-306. https://doi.org/10.1007/s00774-017-0844-5

APA Citation styleDaghma, D., Malhan, D., Simon, P., Stoetzel, S., Kern, S., Hassan, F., Lips, K., Heiss, C., & El Khassawna, T. (2018). Computational segmentation of collagen fibers in bone matrix indicates bone quality in ovariectomized rat spine. Journal of Bone and Mineral Metabolism. 36(3), 297-306. https://doi.org/10.1007/s00774-017-0844-5



Keywords


Collagen fibrilsextracellular matrixOsteoporotic animal modelTransmission electron microscope

Last updated on 2025-18-06 at 13:48