Journal article

Simultaneous and Independent Dual Site-Specific Self-Labeling of Recombinant Antibodies


Authors listWollschlaeger, Carolin; Meinhold-Heerlein, Ivo; Cong, Xiaojing; Braeutigam, Karen; Di Fiore, Stefano; Zeppernick, Felix; Klockenbring, Torsten; Stickeler, Elmar; Barth, Stefan; Hussain, Ahmad Fawzi

Publication year2018

Pages3586-3594

JournalBioconjugate Chemistry

Volume number29

Issue number11

ISSN1043-1802

DOI Linkhttps://doi.org/10.1021/acs.bioconjchem.8b00545

PublisherAmerican Chemical Society


Abstract
Antibody-based diagnostic and therapeutic reagents armed with effector molecules such as dyes and drugs offer hope in the battle against cancer. Several site-specific conjugation methods have been developed to equip antibodies with such effector molecules, but they tend to be expensive and involve multiple reaction steps. The conjugation of two different effector molecules to a single antibody also remains a major challenge. Here we describe a simple, controlled, and robust method for the dual site-specific conjugation of an antibody with two effector molecules in a single-pot reaction using the self labeling SNAP and CLIP protein tags. We verified the principle of the method by labeling an epidermal growth factor receptor (EGFR)-specific single-chain antibody fragment (scFv-425) simultaneously with IRDye700 and Alexa-Fluor647. This dual-labeled antibody bound to EGFR(+) ovarian cancer cell lines and tissue samples with high specificity, and its phototherapeutic efficacy was confirmed by the selective killing of EGFR(+) cells in vitro.



Citation Styles

Harvard Citation styleWollschlaeger, C., Meinhold-Heerlein, I., Cong, X., Braeutigam, K., Di Fiore, S., Zeppernick, F., et al. (2018) Simultaneous and Independent Dual Site-Specific Self-Labeling of Recombinant Antibodies, Bioconjugate Chemistry, 29(11), pp. 3586-3594. https://doi.org/10.1021/acs.bioconjchem.8b00545

APA Citation styleWollschlaeger, C., Meinhold-Heerlein, I., Cong, X., Braeutigam, K., Di Fiore, S., Zeppernick, F., Klockenbring, T., Stickeler, E., Barth, S., & Hussain, A. (2018). Simultaneous and Independent Dual Site-Specific Self-Labeling of Recombinant Antibodies. Bioconjugate Chemistry. 29(11), 3586-3594. https://doi.org/10.1021/acs.bioconjchem.8b00545



Keywords


CAMPTOTHECINCONJUGATIONDOXORUBICINFUSIONMOLECULAR-DYNAMICSMONOCLONAL-ANTIBODY

Last updated on 2025-21-05 at 18:27