Journal article

Publication year: 2018

Pages: 1225-1235

Journal: Osteoarthritis and Cartilage

Volume number: 26

Issue number: 9

ISSN: 1063-4584

eISSN: 1522-9653

Open access status: Bronze

DOI Link: https://doi.org/10.1016/j.joca.2018.06.001

Publisher: Elsevier

Abstract: 

Objectives: Age-related bone loss is associated with bone marrow adiposity. Adipokines (e.g., visfatin, resistin, leptin) are adipocyte-derived factors with immunomodulatory properties and might influence differentiation of bone marrow-derived mesenchymal stem cells (MSC) in osteoarthritis (OA) and osteoporosis (OP). Thus, the presence of adipokines and MMPs in bone marrow and their effects on MSC differentiation were analyzed.

Methods: MSC and ribonucleic acid (RNA) were isolated from femoral heads after hip replacement surgery of OA or osteoporotic femoral neck fracture (FF) patients. Bone structural parameters were evaluated by microcomputed tomography (mCT). MSC were differentiated towards adipocytes or osteoblasts with/without adipokines. Gene expression (adipokines, bone marker genes, MMPs, TIMPs) and cytokine production was evaluated by realtime-polymerase chain reaction (realtime-PCR) and enzymelinked immunosorbent assay (ELISA). Matrix mineralization was quantified using Alizarin red S staining.

Results: mCT showed an osteoporotic phenotype of FF compared to OA bone (reduced trabecular thickness and increased ratio of bone surface vs volume of solid bone). Visfatin and leptin were increased in FF vs OA. Visfatin induced the secretion of IL-6, IL-8, and MCP-1 during osteogenic and adipogenic differentiation. In contrast to resistin and leptin, visfatin increased MMP2 and MMP13 during adipogenesis. In osteogenically differentiated cells, MMPs and TIMPs were reduced by visfatin. Visfatin significantly increased matrix mineralization during osteogenesis, whereas collagen type I expression was reduced.

Conclusion: Visfatin-mediated increase of matrix mineralization and reduced collagen type I expression could contribute to bone fragility. Visfatin is involved in impaired bone remodeling at the adipose tissue/bone interface through induction of proinflammatory factors and dysregulated MMP/TIMP balance during MSC differentiation. (c) 2018 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

Harvard Citation style: Tsiklauri, L., Werner, J., Kampschulte, M., Frommer, K., Berninger, L., Irrgang, M., et al. (2018) Visfatin alters the cytokine and matrix-degrading enzyme profile during osteogenic and adipogenic MSC differentiation, Osteoarthritis and Cartilage, 26(9), pp. 1225-1235. https://doi.org/10.1016/j.joca.2018.06.001

APA Citation style: Tsiklauri, L., Werner, J., Kampschulte, M., Frommer, K., Berninger, L., Irrgang, M., Glenske, K., Hose, D., El Khassawna, T., Pons-Kuehnemann, J., Rehart, S., Wenisch, S., Mueller-Ladner, U., & Neumann, E. (2018). Visfatin alters the cytokine and matrix-degrading enzyme profile during osteogenic and adipogenic MSC differentiation. Osteoarthritis and Cartilage. 26(9), 1225-1235. https://doi.org/10.1016/j.joca.2018.06.001