Journal article

Publication year: 2017

Pages: 49-53

Journal: Transplant Immunology

Volume number: 43-44

ISSN: 0966-3274

eISSN: 2210-3384

DOI Link: https://doi.org/10.1016/j.trim.2017.04.004

Publisher: Elsevier

Abstract: 
This study tested the hypothesis that neuropeptide Y (NPY) and NPY receptors 1 (Yl) and 2 (Y2) participate in lung allograft rejection. Inflammation in grafts may include interaction between blood leukocytes and graft endothelial cells and marked accumulation of intravascular blood leukocytes. Fewer leukocytes accumulate in lung than in kidney allografts. Lung transplantion was performed in the Dark Agouti to Lewis rat strain combination. Intravascular and intraalveolar leukocytes were isolated from the grafts, and we evaluated the mRNA expression of NPY, Yl, and Y2 by real-time RT-PCR as well as the peptide expression of NPY by radio immunoassay and immunohistochemistry. NPY and Yl were expressed by pulmonary intravascular and intraalveolar leukocytes. Yl was up-regulated by pulmonary intravascular and intraalveolar leukocytes during allograft rejection while Y2 could not be detected. Higher NPY expression levels in intravascular leukocytes were observed in lung compared to kidney allografts, which were investigated previously. Our findings suggest that an increased leukocytic expression of NPY in lung compared to kidney allografts results in a reduced accumulation of leukocytes in allograft vessels.

Harvard Citation style: Schmitz, J., Zakrzewicz, A., Wilker, S., Kuncova, J., Hecker, A., Grau, V., et al. (2017) Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts, Transplant Immunology, 43-44, pp. 49-53. https://doi.org/10.1016/j.trim.2017.04.004

APA Citation style: Schmitz, J., Zakrzewicz, A., Wilker, S., Kuncova, J., Hecker, A., Grau, V., Padberg, W., & Holler, J. (2017). Non-neuronal neuropeptide Y and its receptors during acute rejection of rat pulmonary allografts. Transplant Immunology. 43-44, 49-53. https://doi.org/10.1016/j.trim.2017.04.004