Journal article

Publication year: 2017

Pages: 1624-1636

Journal: Inflammatory Bowel Diseases

Volume number: 23

Issue number: 9

ISSN: 1078-0998

eISSN: 1536-4844

DOI Link: https://doi.org/10.1097/MIB.0000000000001159

Publisher: Oxford University Press

Abstract: 

Background: Anti-Glycoprotein 2 (GP2) antibodies are associated with a more complicated course of Crohn's disease (CD) in adults. Four different GP2 isoforms with different length and antibody-binding sites have been identified so far but not been explored in serological studies. We aimed to investigate the diagnostic utility of autoantibodies against all 4 isoforms of GP2 in an exclusively pediatric population for the first time.

Methods: We included 278 children and adolescents with inflammatory bowel disease: 164 with CD, 114 with ulcerative colitis, 83 disease controls (acute gastrointestinal infection, nonspecific gastrointestinal functional disorders), and 219 healthy controls. Sera were tested for anti-GP2 antibodies using 4 different isoforms of GP2 for anti-Saccharomyces cerevisiae antibodies, antineutrophil cytoplasmic antibodies, and pancreatic antibodies.

Results: Anti-GP2 antibodies were significantly more prevalent in patients with CD than in ulcerative colitis and controls. We found a sensitivity of 38% (with a specificity of 95%) for anti-GP2 IgG against isoform 4 in CD. Anti-GP2 IgA against isoform 1 and anti-GP2 IgG against isoform 4 possessed the best diagnostic values for identification of CD. For the differentiation of CD from ulcerative colitis anti-GP2 IgG against isoforms 3 and 4 proved to be most accurate markers. Anti-GP2 antibodies were associated with a more complicated disease behavior and bowel surgery in CD. In a subgroup of patients with CD, anti-GP2 IgG against isoform 4 proved to be a relatively stable marker over time independent of disease activity.

Conclusions: Anti-GP2 antibodies against different isoforms are specific markers for CD and for different phenotypes in pediatric inflammatory bowel disease.

Harvard Citation style: Roeber, N., Noss, L., Goihl, A., Reinhold, D., Jahn, J., de laffolie, J., et al. (2017) Autoantibodies Against Glycoprotein 2 Isoforms in Pediatric Patients with Inflammatory Bowel Disease, Inflammatory Bowel Diseases, 23(9), pp. 1624-1636. https://doi.org/10.1097/MIB.0000000000001159

APA Citation style: Roeber, N., Noss, L., Goihl, A., Reinhold, D., Jahn, J., de laffolie, J., Johannes, W., Flemming, G., Roggenbuck, D., Conrad, K., & Laass, M. (2017). Autoantibodies Against Glycoprotein 2 Isoforms in Pediatric Patients with Inflammatory Bowel Disease. Inflammatory Bowel Diseases. 23(9), 1624-1636. https://doi.org/10.1097/MIB.0000000000001159