Journal article

Publication year: 2017

Pages: 1-12

Journal: Cells Tissues Organs

Volume number: 204

Issue number: 1

ISSN: 1422-6405

eISSN: 1422-6421

Open access status: Bronze

DOI Link: https://doi.org/10.1159/000471794

Publisher: Karger Publishers

Abstract: 
Aim: To introduce embryoid bodies derived from mouse embryonic stem (ES) cells, which differentiate blood vessel-like structures and leukocytes, as a novel in vitro model system for biocompatibility, inflammation, and angiogenesis studies. Methodology/Results: Punched spherical discs of bioabsorbable polymers (epsilon-caprolactone and L-lactide in different compositions) with a diameter of 2 mm and a thickness of 0.2 mm were inoculated with embryoid bodies for cocultivation. As reference material for biocompatible, nonbioabsorbable, and bioincompatible materials, polymer punched discs of petriPERM (PP) membrane (polytetrafluoroethylene) as well as polyvinylchloride (PVC) were used. Tissue outgrowth on the polymer discs decreased and cell toxicity increased upon confrontation on bioabsorbable biomaterials and PVC. Bioabsorbable polymers as well as PVC decreased the branching points and total tube length of CD31-positive vascular structures in embryoid bodies. With the exception of PP, all applied materials increased the differentiation of CD68-positive macrophages and the generation of reactive oxygen species, which is indicative of proinflammatory processes upon contact of tissue with biomaterials. Consequently, cocultivation with polymers increased secretion of the cytokines interleukin-6, monocyte chemotactic protein- 1, and tumor necrosis factor-a. Conclusion: Three-dimensional tissues cultivated from ES cells are well-suited for testing the biocompatibility, the vascular response, and the inflammatory reaction towards bioabsorbable and nonbioabsorbable polymers. (C) 2017 S. Karger AG, Basel

Harvard Citation style: Sharifpanah, F., Reinhardt, M., Schoenleben, J., Meyer, C., Richter, M., Schnabelrauch, M., et al. (2017) Embryonic Stem Cells for Tissue Biocompatibility, Angiogenesis, and Inflammation Testing, Cells Tissues Organs, 204(1), pp. 1-12. https://doi.org/10.1159/000471794

APA Citation style: Sharifpanah, F., Reinhardt, M., Schoenleben, J., Meyer, C., Richter, M., Schnabelrauch, M., Rode, C., Wartenberg, A., Bekhite, M., Sauer, H., & Wartenberg, M. (2017). Embryonic Stem Cells for Tissue Biocompatibility, Angiogenesis, and Inflammation Testing. Cells Tissues Organs. 204(1), 1-12. https://doi.org/10.1159/000471794