Journal article
Authors list: Krumbholz, G.; Junker, S.; Meier, F. M. P.; Rickert, M.; Steinmeyer, J.; Rehart, S.; Lange, U.; Frommer, K. W.; Schett, G.; Mueller-Ladner, U.; Neumann, E.
Publication year: 2017
Pages: 406-414
Journal: Clinical and Experimental Rheumatology
Volume number: 35
Issue number: 3
ISSN: 0392-856X
eISSN: 1593-098X
Publisher: Clinical and Experimental Rheumatology Sas
Objective Adiponectin is an effector molecule in the pathophysiology of rheumatoid arthritis, e.g. by inducing cytokines and matrix degrading enzymes in synovial fibroblasts. There is growing evidence that adiponectin affects osteoblasts and osteoclasts although the contribution to the aberrant bone metabolism in rheumatoid arthritis is unclear. Therefore, the adiponectin effects on rheumatoid arthritis-derived osteoblasts and osteoclasts were evaluated. Methods Adiponectin and its receptors were examined in bone tissue. Primary human osteoblasts and osteoclasts were stimulated with adiponectin and analysed using realtime polymerase chain-reaction and immunoassays. Effects on matrix-production by osteoblasts and differentiation and resorptive activity of osteoclasts were examined. Results Immunohistochemistry of rheumatoid arthritis bone tissue showed adiponectin expression in key cells of bone remodelling. Adiponectin altered gene expression and cytokine release in osteoblasts and increased IL-8 secretion by osteoclasts. Adiponectin inhibited osterix and induced osteoprotegerin mRNA in osteoblasts. In osteoclasts, MMP-9 and tartrate resistant acid phosphatase expression was increased. Accordingly, mineralisation capacity of osteoblasts decreased whereas resorptive activity of osteoclasts increased. Conclusion The results confirm the proinflammatory potential of adiponectin and support the idea that adiponectin influences rheumatoid arthritis bone remodelling through alterations in osteoblast and osteoclast.
Abstract:
Citation Styles
Harvard Citation style: Krumbholz, G., Junker, S., Meier, F., Rickert, M., Steinmeyer, J., Rehart, S., et al. (2017) Response of human rheumatoid arthritis osteoblasts and osteoclasts to adiponectin, Clinical and Experimental Rheumatology, 35(3), pp. 406-414
APA Citation style: Krumbholz, G., Junker, S., Meier, F., Rickert, M., Steinmeyer, J., Rehart, S., Lange, U., Frommer, K., Schett, G., Mueller-Ladner, U., & Neumann, E. (2017). Response of human rheumatoid arthritis osteoblasts and osteoclasts to adiponectin. Clinical and Experimental Rheumatology. 35(3), 406-414.
Keywords
ADIPOCYTOKINES; BONE-RESORPTION; JOINT DESTRUCTION; OSTEOBLASTS; OSTEOCLASTS; TRANSCRIPTION FACTOR OSTERIX