Journal article

Publication year: 2017

Pages: 114-117

Journal: Veterinary Microbiology

Volume number: 200

ISSN: 0378-1135

eISSN: 1873-2542

DOI Link: https://doi.org/10.1016/j.vetmic.2015.09.001

Publisher: Elsevier

Abstract: 
Carbapenem-resistant Enterobacteriaceae are not any more isolated only from human settings, but also from livestock. We reported for the first time the presence of VIM-1 carbapenemases in a livestock farm in Germany. The VIM-1 resistance gene found in these farms was located on IncHI2 plasmids. In order to be able to analyse these plasmids in more detail, two different plasmids from a single farm (pRH-R27 from Salmonella enterica and pRH-R178 from Escherichia colt) were completely sequenced and analysed for the presence of antibiotic and heavy metal resistances. The plasmids showed to harbour bla(VIM-1), aacA4, aadA1, sul1, qacE Delta (encoded in an In110 class 1 integron), as well as bla(ACC-1), strA/strB, and catA1 genes together with resistance to heavy metals (ter-, mer-, sil-, ars-, rcn-, and pco). Comparison with other IncHI2 plasmid revealed that while pRH-R27 is a mosaic IncHI2 plasmid with both high homology to the plasmid pSTm-A54650 and R478, both isolated from humans, pRH-R178 is a deletion derivative of pRH-R27, presumably caused by several IS-mediated deletions indicating genetic evolution of plasmids in this environment. (C) 2015 Elsevier B.V. All rights reserved.

Harvard Citation style: Falgenhauer, L., Ghosh, H., Guerra, B., Yao, Y., Fritzenwanker, M., Fischer, J., et al. (2017) Comparative genome analysis of IncHI2 VIM-1 carbapenemase-encoding plasmids of Escherichia coli and Salmonella enterica isolated from a livestock farm in Germany, Veterinary Microbiology, 200, pp. 114-117. https://doi.org/10.1016/j.vetmic.2015.09.001

APA Citation style: Falgenhauer, L., Ghosh, H., Guerra, B., Yao, Y., Fritzenwanker, M., Fischer, J., Helmuth, R., Imirzalioglu, C., & Chakraborty, T. (2017). Comparative genome analysis of IncHI2 VIM-1 carbapenemase-encoding plasmids of Escherichia coli and Salmonella enterica isolated from a livestock farm in Germany. Veterinary Microbiology. 200, 114-117. https://doi.org/10.1016/j.vetmic.2015.09.001