Journalartikel

Jahr der Veröffentlichung: 2016

Seiten: 4329-4337

Zeitschrift: The Journal of Clinical Endocrinology & Metabolism

Bandnummer: 101

Heftnummer: 11

ISSN: 0021-972X

eISSN: 1945-7197

Open Access Status: Bronze

DOI Link: https://doi.org/10.1210/jc.2016-1754

Verlag: Oxford University Press

Abstract: 

Context: The profile of urinary steroids as measured by gas chromatography-mass spectrometry defines a subject's "steroidal fingerprint."

Objective: Here, we clustered steroidal fingerprints to characterize patients with nonsyndromic childhood obesity by "steroid metabolomic signatures."

Hypothesis: Nonsyndromic obesity is a symptom of different diseases and conditions, some of them will have their own signature.

Design: A total of 31 steroid metabolites were quantified by gas chromatography-mass spectrometry, and their excretion rates were z-transformed. Using MetaboAnalyst 3.0, we divided the subjects into 5 distinctive groups by k-means clustering. Steroidal fingerprints and clinical/biochemical data of patients in each cluster were analyzed.

Patients: A total of 87 obese children (44 females), aged 8.5-17.9 years, were clinically characterized, and their 24-hour urine was collected.

Results: Cluster 1 (n = 39, 21 females) had normal steroid profile. Cluster 2 (n = 20, 11 females) showed mild, nonspecific elevation of C19 and C21 steroids, females' resistance to polycystic ovary morphology, and hirsutism. Cluster 3 (n = 7 female), with relative 21-hydroxylase insufficiency, was characterized by partial or full polycystic ovary syndrome. Cluster 4 (n = 4 males), showed markedly elevated C21 steroids and imbalance in the 11 beta-hydroxysteroid dehydrogenase system, higher insulin, increased frequency of glucose/insulin index more than 0.3, gamma-glutamyl transpeptidase activity, systolic blood pressure, and tendency to liver steatosis. Cluster 5 (n = 17, 5 females) had elevated dehydroepiandrosterone and 17-OH-pregnenolone metabolites, suggesting 3 beta-hydroxysteroid dehydrogenase insufficiency but no clinically unique phenotype. Z-score body mass index values were not significantly different between the clusters.

Conclusions: We defined a novel concept of disease-specific steroid metabolomic signature based on urinary steroidal gas chromatography-mass spectrometry. Clustering by software designed for metabolic data analysis reclassified childhood obesity into 5 groups with distinctive signatures; groups require further definition and may require cluster-specific therapeutic strategies.

Harvard-Zitierstil: Gawlik, A., Shrnoish, M., Hartmann, M., Malecka-Tendera, E., Wudy, S. and Hochberg, Z. (2016) Steroid Metabolomic Disease Signature of Nonsyndromic Childhood Obesity, The Journal of Clinical Endocrinology & Metabolism, 101(11), pp. 4329-4337. https://doi.org/10.1210/jc.2016-1754

APA-Zitierstil: Gawlik, A., Shrnoish, M., Hartmann, M., Malecka-Tendera, E., Wudy, S., & Hochberg, Z. (2016). Steroid Metabolomic Disease Signature of Nonsyndromic Childhood Obesity. The Journal of Clinical Endocrinology & Metabolism. 101(11), 4329-4337. https://doi.org/10.1210/jc.2016-1754