Journal article

Publication year: 2016

Pages: 72-77

Journal: Clinica Chimica Acta

Volume number: 460

ISSN: 0009-8981

eISSN: 1873-3492

DOI Link: https://doi.org/10.1016/j.cca.2016.06.023

Publisher: Elsevier

Abstract: 

Background: Immunofluorescence assays of antibodies against endomysium (EmA) on primate oesophagus sections represent the gold standard in serological testing for coeliac disease (CD). As alternative immunofluorescence technique, staining of primate liver tissue is in use. We compared performance and predictive power of IgA- and IgG-EmA on primate oesophagus and primate liver sections.

Methods: Sera of 298 paediatric biopsy-proven CD patients under gluten-containing diet and 574 disease controls were investigated. Samples were collected between 2004 and 2013 in six children's hospitals. The antibodies were assayed blinded to diagnoses and histological data. Sensitivity, specificity, and positive (PPV) and negative predictive values (NPV) were calculated for different assays.

Results: (Oesophagus vs liver): For IgA-EmA, sensitivity (0.953 vs 0.956) and specificity (0.981 vs 0.972) as well as PPV (0.963 vs 0.947) and NPV (0.976 vs 0.979) were comparable on both tissues. IgG-EmA on liver showed significantly higher sensitivity (0.520 vs 0.631; p = 0.006) but significantly lower specificity (0.995 vs 0.963; p = 0.002) and PPV (0.981 vs 0.899; p = 0.0002) than on oesophagus. NPV on liver was higher than NPV on oesophagus, however, the difference was not statistically significant (0.799 vs 0.834; p = 0.099).

Conclusion: Primate liver can be used as alternative, equally well functioning substrate for IgA-EmA testing. (C) 2016 Elsevier B.V. All rights reserved.

Harvard Citation style: Wolf, J., Jahnke, A., Fechner, K., Richter, T., Laass, M., Hauer, A., et al. (2016) Primate liver tissue as an alternative substrate for endomysium antibody immunofluorescence testing in diagnostics of paediatric coeliac disease, Clinica Chimica Acta, 460, pp. 72-77. https://doi.org/10.1016/j.cca.2016.06.023

APA Citation style: Wolf, J., Jahnke, A., Fechner, K., Richter, T., Laass, M., Hauer, A., Stern, M., de Laffolie, J., Flemming, G., & Mothes, T. (2016). Primate liver tissue as an alternative substrate for endomysium antibody immunofluorescence testing in diagnostics of paediatric coeliac disease. Clinica Chimica Acta. 460, 72-77. https://doi.org/10.1016/j.cca.2016.06.023