Journalartikel

Jahr der Veröffentlichung: 2016

Seiten: 1048-1054

Zeitschrift: Biosensors and Bioelectronics

Bandnummer: 77

ISSN: 0956-5663

eISSN: 1873-4235

DOI Link: https://doi.org/10.1016/j.bios.2015.10.076

Verlag: Elsevier

Abstract: 

The response characteristics of acetylcholinesterase-modified AlGaN/GaN solution-gate field-effect transistors (AcFETs) are quantitatively analyzed by means of a kinetic model. The characterization shows that the covalent enzyme immobilization process yields reproducible AcFET characteristics with a Michaelis constant K-M of (122 +/- 4) mu M for the immobilized enzyme layer. The increase of K-M by a factor of 2.4 during the first four measurement cycles is attributed to partial denaturation of the enzyme. The AcFETs were used to record the release of acetylcholine (ACh) by neuronal tissue cultivated on the gate area upon stimulation by rising the extracellular K+ concentration. The neuronal tissue constituted of isolated myenteric neurons from four to 12 days old Wistar rats, or sections from the muscularis propria containing the myenteric plexus from adult rats. For both cases the AcFET response was demonstrated to be related to the activity of the immobilized acetylcholinesterase using the reversible acetylcholinesterase blocker donepezil. A concentration response curve of this blocking agent revealed a half maximal inhibitory concentration of 40 nM which is comparable to values measured by complementary in vitro methods.

Harvard-Zitierstil: Müntze, G., Pouokam, E., Steidle, J., Schäfer, W., Sasse, A., Röth, K., et al. (2016) In situ monitoring of myenteric neuron activity using acetylcholinesterase-modified AlGaN/GaN solution-gate field-effect transistors, Biosensors and Bioelectronics, 77, pp. 1048-1054. https://doi.org/10.1016/j.bios.2015.10.076

APA-Zitierstil: Müntze, G., Pouokam, E., Steidle, J., Schäfer, W., Sasse, A., Röth, K., Diener, M., & Eickhoff, M. (2016). In situ monitoring of myenteric neuron activity using acetylcholinesterase-modified AlGaN/GaN solution-gate field-effect transistors. Biosensors and Bioelectronics. 77, 1048-1054. https://doi.org/10.1016/j.bios.2015.10.076